银杏叶提取物预防离体大鼠心肌缺血再灌注损伤

探讨银杏叶提取物在心肌缺血再灌注损伤中的预防作用。方法取３０只ＳＤ大鼠平均为３组，采用改良Ｌａｎｇｅｎｄｏｒｆｆ法建立离体大鼠心脏缺血再灌注模型，以Ｋｒｅｂｓ－Ｈｅｎｓｅｌｅｉｔ碳酸氢盐酸冲液（ＫＨＢＢ）灌注１０ｍｉｎ后，实验组和对照组分别予以Ｅｇｂ７６１加晶体停搏液或晶体停搏液停搏，停搏６０ｍｉｎ后再以ＫＨＢＢ缓冲灌注６０ｍｉｎ，在复灌１５、３０、６０ｍｉｎ时点测定冠脉量，心率、左心室压力，心收

In vitro preventive effect of Ginkgo biloba extracts on myocardial ischemic reperfusive injury in SD rats

AIM: To study the in vitro preventive effect of Ginkgo biloba extracts (Egb761) on myocardial ischemic reperfusive injury in SD rats. METHODS: Thirty SD rats, which were divided into 3 groups (n 1=n 2=n 3=10), were chosen to make the model of myocardial ischemic reperfusive injury of SD rats by using developed langendorff methods. After 10 min of perfusion with Krebs Henseleit bicarbonate buffer (KHBB), the hearts of SD rats in the test group were arrested by the cardioplegia with Egb761, while those in the placebo group were arrested only by the cardioplegia without Egb761. Sixty min later, all the hearts of SD rats were reperfused by the KHBB, and the coronary flow, heart rate, left ventricular pressure were measured at 15, 30, 60 min after reperfusion, the perfusive fluid of the hearts were collected to measure the value of lactate dehydrogenase (LDH) and creatine phosphokinase (CPK). The myocardia of the rats were tested by the electron microscope. RESULTS: The coronary flow decreased both in the test group and in the placebo group after the early reperfusion, but it recovered more quickly and more significantly in the test group (P<005). The values of LDH and CPK in the test group were lower than those in the placebo group (P<001,P<0001) and the changes of the myocardial ultramicrostructure were less significant in the test group. CONCLUSION: It is suggested that Egb761 could prevent the myocardial ischemic reperfusive injury in SD rats.