湖南籍汉族女性HLA—A／B单倍型分析

目的：调查分析湖南籍汉族女性人群中HLA－A，B位点单倍型基因频率。方法：采用微量淋巴细胞毒试验法对50例无血缘关系的湖南籍汉族女性HLA－A，B位点多态性进行检查，通过比较连锁不平衡参数来推测单倍型。结果：居单倍型基因频率前3位的是A11－B60，A2－B60，A24－B48，而A2－B52，A1－B13，A24－B52等5种单倍型有显著的连锁不平衡，结论：湖南籍汉族女性HLA－A，B位点抗原，特别是B抗原的分布与国内其它地区汉族人群及湖南地区汉族人群（男女混合）比较有其特殊性，A2－B52，A11－B13，A24－B52三种单倍型有显著的连锁不平衡，可能为湖南籍汉族女性特有的单倍型。

Analysis of the HLA A/B local haplotype among the females of Han nationality in Hunan province]

OBJECTIVE: To investigate and analyse HLA A/B local haplotype gene frequency (HGF) in the females of Han nationality in Hunan. METHODS: HLA-A, B local antigen polymorphisms were investigated via microcytotoxicity assay. Haplotype was inferred by means of linkage disequilibrium parameter. RESULTS: Among the 46 haplotypes, HGF high were: A11-B60 (HGF = 0.1381), A2-B60 (HGF = 0.0861), A24-B48 (HGF = 0.0709). Three haplotype's linkage disequilibrium parameter have significant difference, they are A2-B52, A11-B13, A24-B52. CONCLUSION: The female of Han Nationality of Hunan HLA-A, B especially B local antigen frequency exists difference sompare with Han Nationality in Hunan and other areas in China of mix public of both sexes. When study relativity of the female disease of Han Nationality of Hunan with HAL, should notice HLA distributing particularity, be assure data veracity. Among the 46 haplotypes, A2-B52, A11-B13, A24-B52 three haplotypes exist significant linkage disequilibrium, probably they are own haplotypes at the female of Han Nationality in Hunan.