蛋白聚糖Versican V1区诱导小鼠脊柱炎和骶髂关节炎模型的建立

目的 建立自身免疫性强直性脊柱炎(AS)实验动物模型，并探讨重组人蛋白聚糖Versican的G1区多肽(VG1)在其脊柱和骶髂关节炎发病中的作用。方法 用自然折叠的VG1和完全弗氏佐剂免疫BALB／c小鼠，诱导产生脊柱炎和骶髂关节炎，建立自身免疫性AS实验动物模型。采用组织病理学方法显示脊柱炎小鼠的发病程度和病理学特征。结果 用VG1和完全弗氏佐剂免疫的实验组小鼠脊柱炎和骶髂关节炎的发病率分别为35．0％和12．5％，未出现外周炎的临床症状。组织病理学结果显示，脊柱韧带与椎间盘连接处、椎间盘和骶髂关节有大量的单个核细胞浸润。结论 成功建立了VGl诱导的小鼠脊柱炎模型，并发现针对蛋白聚糖Versican的免疫反应参与了BALB／c小鼠脊柱炎和骶髂关节炎的发病，为进一步深入研究人类AS的发病机制及其临床治疗提供了有价值的实验材料。

Induction of spondylitis and sacroiliitis in mice by the G1 domain of the proteoglycan versican

Objective To set up rodent ankylosing spondylitis model. Methods Recombinant natively folded versican G1 domain(VG1) and FCA were injected into BALB/c mice to induce spondylitis and sacroiliitis. Spondylitis and sacroiliitis were determined by pathological examination. Results It was shown that immunity to recombinant VG1 resulted in spondylitis in the lumbar spine and sacroiliitis in 35 0% and 12 5% mice respectively. Accumulation of mononuclear cells was observed in spinal ligaments adjacent to the intervertebral disc, the intervertebral disc and the sacroiliac joints. No clinical peripheral arthritis was observed. Conclusion These observations suggest that immunity to human VG1 is involved in the induction of experimental spondylitis and sacroiliitis in BALB/c mice, which will do some help to probe the pathogenesis of human ankylosing spondylitis