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| 体外培养版纳微型猪近交系骨髓基质干细胞的生物学特性 | |
| 引用本文： | 李胜富,卢晓风,孙明菡,万琳,步宏,曾养志,周宏治,李幼平.体外培养版纳微型猪近交系骨髓基质干细胞的生物学特性[J].中国修复重建外科杂志,2002,16(5):354-358. |
| 作者姓名： | 李胜富 卢晓风 孙明菡 万琳 步宏 曾养志 周宏治 李幼平 |
| 作者单位： | 1. 四川大学华西医院卫生部移植工程与移植免疫重点实验室,成都,610041 2. 云南农业大学遗传资源实验室 3. 四川教育学院生物系 |
| 基金项目： | 国家自然科学基金重大项目 (39993430 )，国家重点基础研究发展规划(973) (G1 9990 6470 1 )，美国纽约中华医学基金会资助项目(CMB 2 0 0 1 96636)，教育部博士点基金资助项目(2 0 0 0 53)~~ |
| 摘 要： | 目的 分离培养成年版纳微型猪近交系骨髓基质干细胞并探讨其体外生物学特性。方法 成年版纳微型猪骨髓基质干细胞通过密度梯度离心法分离获得 ,用含 15 %小牛血清的 DMEM在 37℃ ,5 % CO2 条件下培养 ;通过克隆生长特性、特异抗原表达和成骨分化能力鉴定其干细胞特性。结果 版纳微型猪近交系骨髓分离获得的基质干细胞具有多种形状 ,但主要为梭形。其具有很强的增殖能力 ,在体外培养条件下可以观察到明显的克隆样生长 ;表达 SH2、SH3、SH4、SB10和 SB2 1等骨髓基质干细胞的特异标记 ;经成骨添加剂诱导培养之后能分化为成骨细胞 ,碱性磷酸酶活性增强并具有细胞外基质矿化能力。结论 成年版纳微型猪近交系骨髓来源的基质干细胞具有干细胞的一般生物学特性 |
| 关 键 词： | 骨髓基质干细胞 成骨细胞 版纳微型猪近交系 |
| 修稿时间： | 2002年3月28日 |
BIOLOGICAL CHARACTERISTICS OF MESENCHYMAL STEM CELLS IN VITRO DERIVED FROM BONE MARROW OF BANNA MINIPIG INBRED LINE |
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| LI Sheng fu,LU Xiao feng,SUN Ming han,et al..BIOLOGICAL CHARACTERISTICS OF MESENCHYMAL STEM CELLS IN VITRO DERIVED FROM BONE MARROW OF BANNA MINIPIG INBRED LINE[J].Chinese Journal of Reparative and Reconstructive Surgery,2002,16(5):354-358. | |
| Authors: | LI Sheng fu LU Xiao feng SUN Ming han |
| Institution: | Key Laboratory of Transplant Engineering and Immunology, Ministry of Public Health, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041. lishengfu@163.net |
| Abstract: | OBJECTIVE: To isolate and characterize mesenchymal stem cells (MSCs) derived from bone marrow of Banna minipig inbred line (BMI). METHODS: BMI-MSCs was isolated from bone marrow by density gradient centrifugation and cultured in DMEM (containing 15% bovine serum) at 37 degrees C with humidified 5% CO2. These cultured stem cells were characterized in clonal growth, expression of specific markers and capability of differentiation. RESULTS: Mesenchymal stem cells were proliferative and could be expanded rapidly in vitro. Clonal growth of these cells can be observed when small amount of cells was inoculated. These cells were SH2, SH3, SH4, SB10 and SB21 positive. And it was proved that these cells possess osteo-differentiation ability, up-regulated alkaline phosphatase expression and calcium secretion after osteosupplement was added into the media for several days. CONCLUSION: Mesenchymal stem cells derived from bone marrow of BMI possess the general characters of stem cell. |
| Keywords: | Marrow mesenchymal stem cells Osteoblasts Banna minipig inbred line |
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