纤维素三醋酸酯手性固定相分离药物对映体

用非均相乙酰化方法合成微晶纤维素三醋酸酯制备手性柱和手性薄层板用于HPLC和TLC,用95%乙醇或95%乙醇和缓冲液(pH10)的混合物为流动相分离特罗格尔碱、甲喹酮、氯美扎酮和氯喹对映体,讨论了流动相组成、pH、温度对分离对映体的影响。实验结果表明CTA手性板对流动相的组成和pH的变化较敏感,TLC流动相极性一般较HPLC流动相的极性大。上述4种对映体的HPLC分离度均达到0.6以上;TLC分离度均达到1.1以上。

SEPARATION OF PHARMACEUTICAL ENANTIOMERS ON COLUMN AND THIN-LAYER PLATE OF CELLULOSE TRIACETATE

Microcrystalline cellulose triacetate (CTA) was prepared by heterogeneous acetylation of microcrystalline cellulose and used as liquid chromatographic chiral stationary phase and chiral thin layer plate to separate pharmaceutical enantiomers. The racemic Troegor's base, mathaqualone, chlormezanone and chloroquine were separated on the CTA chiral column and on the CTA chiral plate. 95% ethanol and the mixtures of 95% ethanol and water with different ratio and different pH values were used as mobile phase. The influences of eluent composition, pH and temperature on the chiral separation were discussed. The experimental results indicate that the CTA chiral plate was more sensitive to eluent composition and pH than chiral column possibly because the chiral adsorbent was not swollen on the plate.