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Matrix metalloproteinase-2 expression induced by two different adhesive systems on human pulp fibroblasts |
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| Authors: | Orsini Giovanna Mazzoni Annalisa Orciani Monia Putignano Angelo Procaccini Maurizio Falconi Mirella Pashley David H Tay Franklin R Breschi Lorenzo |
| Affiliation: | ∗ Department of Clinical Sciences and Stomatology, Marche Polytechnic University, Ancona, Italy § Department of Clinical and Molecular Sciences, Marche Polytechnic University, Ancona, Italy † Department of Anatomical Sciences, University of Bologna, Bologna, Italy ‡ Laboratory of Cell Biology and Laboratory of Immunorheumatology and Tissue Regeneration-Ramses Laboratory, Rizzoli Orthopaedic Institute, Bologna, Italy ‖ Department of Oral Biology, College of Dental Medicine, Georgia Health Sciences University, Augusta, Georgia ¶ Department of Endodontics, College of Dental Medicine, Georgia Health Sciences University, Augusta, Georgia # Department of Medical Sciences, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste and Istituto di Genetica Molecolare-Consiglio Nazionale Ricerche, Unit of Bologna, Istituti Ortopedici Rizzoli, Bologna, Italy |
| Abstract: | IntroductionThis study evaluated the expression of matrix metalloproteinase-2 (MMP-2) in primary cultures of human pulp fibroblasts (HPFs) when exposed to extracts from dentin-bonding systems.MethodsPolymerized resin disks of the bonding agent of a 2-step self-etch adhesive (TechBond, Isasan, Rovello Porro, Italy) or of the primer/bonding agent a 2-step etch-and-rinse adhesive (Optibond Solo; Sybron-Kerr, Orange, CA) were immersed in HPF culture medium for 24 or 96 hours. HPFs were incubated in the adhesive-conditioned or control (untreated) culture medium for 24 hours. Western blot and immunofluorescence analyses were performed to assay MMP-2 expression.ResultsMMP-2 expression levels in HPFs cultured for 24 hours in culture medium were similar in both the control and experimental media groups showing a faint band at 67 kDa. Conversely, the HPFs incubated in the medium that contain polymerized resin disks for 96 hours showed increased MMP-2 expression compared with the untreated medium. The self-etch adhesive displayed the most pronounced induction of MMP-2 expression. These findings were confirmed by immunofluorescence analysis.ConclusionsHPFs display increased MMP-2 expression after 96 hours of conditioning of the HPF culture medium with polymerized disks of dentin bonding systems. This MMP-2 expression/activation may represent a defence mechanism exhibited by HPFs towards monomers eluted from the dentin bonding systems. |
| Keywords: | Dentin bonding systems fibroblasts matrix metalloproteinase-2 |
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