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| 二仙汤及其组方中药对幼年大鼠卵巢形态学影响 | |
| 引用本文： | 陶仕英,牛建昭,杨美娟,赵丕文,王继峰. 二仙汤及其组方中药对幼年大鼠卵巢形态学影响[J]. 中华中医药杂志, 2010, 0(12) |
| 作者姓名： | 陶仕英 牛建昭 杨美娟 赵丕文 王继峰 |
| 作者单位： | 北京中医药大学基础医学院; |
| 基金项目： | 国家自然科学基金项目(No.30772849); 高等学校创新引智计划项目(No.B07007); 北京中医药大学科研基金(No.0100013)~~ |
| 摘 要： | 目的:通过二仙汤及其组方中药对幼年大鼠卵巢的形态学观察,探讨其对卵巢功能的影响机制。方法:幼年SD大鼠54只,分为正常对照组、阳性对照组、二仙汤组、淫羊藿组、仙茅组、巴戟天组、当归组、黄柏组和知母组9组。各组给药6d后麻醉大鼠,取血清测量卵泡刺激素(FSH)和雌二醇(E2)的含量,分离摘取双侧卵巢,称重后常规石蜡包埋切片,行HE染色和免疫组织化学染色。结果:阳性对照组、仙茅组和黄柏组卵巢系数与正常组对比增加明显,差异有统计学意义(P<0.05)。仅阳性对照组E2含量升高。HE染色发现阳性对照组、二仙汤组、淫羊藿组、仙茅组、当归组和黄柏组卵泡数量明显增多,其中阳性对照组、仙茅组和黄柏组可见黄体形成。免疫组织化学染色发现雌激素受体α(ERα)在各组大鼠的卵巢颗粒细胞中未见表达,雌激素受体β(ERβ)主要表达于各组大鼠的卵巢颗粒细胞的细胞核中;二仙汤组、淫羊藿组、仙茅组、当归组和黄柏组ERβ表达显著增强(P<0.05)。结论:二仙汤及其方中温肾药(淫羊藿、仙茅),滋阴药(黄柏)和调理冲任药(当归)通过上调卵泡颗粒细胞ERβ表达,促进卵泡发育成熟和排卵。 |
| 关 键 词： | 二仙汤 卵巢 雌激素受体 大鼠 |
The morphology observation to ovarian of Development Rats through Er-xian Decoction and its compositions |
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| TAO Shi-ying,NIU Jian-zhao,YANG Mei-juan,ZHAO Pi-wen,WANG Ji-feng. The morphology observation to ovarian of Development Rats through Er-xian Decoction and its compositions[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2010, 0(12) | |
| Authors: | TAO Shi-ying NIU Jian-zhao YANG Mei-juan ZHAO Pi-wen WANG Ji-feng |
| Affiliation: | TAO Shi-ying,NIU Jian-zhao,YANG Mei-juan,ZHAO Pi-wen,WANG Ji-feng (School of Preclinical Medicine,Beijing University of Traditional Chinese Medicine,Beijing 100029,China) |
| Abstract: | Objective: To research their possible functional mechanism through observation the morphology effects of Er-xian Decoction and its compositions on ovarian of Development Rats.Methods:Fifty-four development female SD rats were randomly divided into nine groups:normal control group,positive control group,Er-xian Decoction group,Herba Epimedii group,Rhizoma Curculiginis group,Radix Morindae Officinalis group,Radix Angelicae Sinensis group,Cortex Phellodendri group,Rhizoma Anemarrhenae group.After the rats were... |
| Keywords: | Er-xian Decoction Ovary Estrogen receptor Rat |
| 本文献已被 CNKI 等数据库收录！ | |