HPLC测定妇洗康洗剂中盐酸小檗碱含量

目的建立测定妇洗康洗剂中盐酸小檗碱含量的高效液相色谱法。方法色谱条件：Eclipse XDB C18柱（4.6mm×150mm,5μm）,流动相：乙腈∶水（55∶45）（每1000mL中含5.4g磷酸二氢钾和3.4g十二烷基硫酸钠）,检测波长：345nm,柱温：室温。结果盐酸小檗碱进样量在0.0588μg～0.3528μg范围内线性关系良好（r=0.9997）。平均回收率为100.65%,RSD为1.58%。结论该方法简便、准确,可用于妇洗康洗剂中盐酸小檗碱含量的测定。

Determination of berberina Hydrochloridein Compound Fuxikang lotion by HPLC

OBJECTIVE To establish a method for the determination of berberine hydrochloride in Compound Fuxikang Lotion.METHODS Chromatographic condition：Eclipse XDB C18（4.6mm×150mm,5μm）,Mobile Phase：acetonitrile：water（55∶45）（Each 1000mL contains 5.4g of potassium dihydrogen phosphate and 3.4g sodium lauryl sulfate）,detection wavelength：345nm,column temperature：room temperature.RESULTS The range of berberine hydrochloride was 0.0588ug～0.3528ug where the sample sizes in good linear relationship with peak areas（r=0.9997）.The average recovery reached 100.65%,RSD at 1.58%.CONCLUSION The established method is simple,accurate,thus can be applied to the determination of berberine in Compound Fuxikang Lotion.