蒺藜总皂苷对缺氧复氧心肌细胞NF-κB核移位及其调控下游基因表达的影响

目的观察蒺藜总皂苷(GSTT)对模拟缺血再灌注心肌细胞核因子-κB(NF-κB)核移位及其调控下游基因TNF-α、IL-1β蛋白表达的影响。方法利用原代培养的SD乳鼠心肌细胞建立模拟心肌缺血再灌注模型,分为正常组、模型组和GSTT大、小剂量组。放免法检测TNF-αI、L-1β,免疫组化定性观察NF-κB p65核移位。结果与模型组比较,GSTT大、小剂量组TNF-α、IL-1β含量显著降低(P<0.01或0.05),NF-κB p65核移位明显。结论GSTT对模拟缺血再灌注损伤心肌细胞有保护作用,可减轻炎症损伤,其机制与上游调控枢纽核转录因子NF-κB激活有关。

Influence of GSTT on NF- κB activity and its downstream gene expression in cardiac muscle cell with hypoxia- reoxygenation

Objective It is to observe the influence of GSTT on NF-κB activity and its downstream gene(TNF-α and IL-1β) expression in cardiac muscle cell with mimic myocardial ischemia-reperfusion.Methods The mimic myocardial ischemia-reperfusion models were made with the cardiac muscle cell of the first generation cultured SD neonate rats and were divided into normal group,model group,large dosage GSTT group and low dosage GSTT group.TNF-α and IL-1β were examined with radioimmunoassay.NF-κB p65 activity was qualitatively observed with immunohistochemistry.Results Compared with model group,the contents of TNF-α and IL-1β were significantly decreased(P<0.01 or 0.05) and NF-κB p65 activity was improved in large dosage GSTT group and low dosage GSTT group.Conclusion GSTT has protection on cardiac muscle cell with mimic myocardial ischemia-reperfusion and can lighten inflammatory injury.The mechanism may be related to the activation of the upstream regulatory pivot nuclear factor NF-κB.