Transformation of ψ
− Saccharomyces cerevisiae to ψ
+ with DNA co-purified with 3 μm circles |
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| Authors: | Hwa Dai S H Tsay P M Lund B S Cox |
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| Institution: | (1) Institute of Botany, Academia Sinica, Taipei, Republic of China;(2) Botany School, South Parks Road, Oxford, UK |
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| Abstract: | Summary DNA enriched for supercoiled plasmids prepared from the 3  m plasmid-enriched, 
+], 2 m°] strain 6-1G-P188 and from the 2 m+] +] strain LL20 can be used to transform a
– recipient strain to
+. Fractionation of the former preparation by electrophoresis showed that the 3 Mm plasmid band contained the transforming activity. |
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| Keywords: | Psi-factor 3-micron plasmids Yeast |
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