三类Ig抗甲状腺球蛋白独特型抗体的检测与意义

用兔抗人甲状腺球蛋白(ATG)包被,建立了检测IgG 类、IgA 类、IgM 类抗TG 独特型抗体(ATG—Ab_2)的方法,此法可不受类风湿因子和TG 与抗TG 免疫复合物(TG—IC)的干扰。以正常对照组A 值的(?)+3S 为正常上限,甲亢、桥本氏甲状腺炎、甲减患者的ATG—Ab_2总阳性率分别为58.2%、100.0%、与40.0%。三类Ig的ATG—Ab_2均存在。对甲亢患者检测的结果表明,ATG—Ab_2与ATG 相互间具有排他性,提示前者对ATG 的产生可能有负调节作用。

The determination of 3 class-specific antithyroglobulin antiidiotypie antibody and its clinical significance

The microtitration plates were coated with rabbit anti-hTG antibody and indirected ELISA was establishedto assay IgG,IgA,IgM class-specific antithyroglobulin antiidiotypic antibody(ATG-Ab2).This method wasnot intenten with RF and TG-IC.The upper limit of nomal is (?)+3SDof nomal subjects absorbance.The totalpositive rate of ATG-Ab2 in sera of the patients with Grave's disease,Hasimoto disease and Hypothyroidismwere 58.2%,100%,40.0% respectively.3 class-specific ATG-Ab2 were all existed in patients sera.The assayingresults of Grave's disease indicated that there may be down regulation effect of ATG-Ab2 on ATG.