| 188Re-DTPA-DG致MCF-7乳腺癌细胞凋亡实验研究 |
| |
| 引用本文： | 熊青峰,陈跃,何菱,黄占文,李举联,邓存良. 188Re-DTPA-DG致MCF-7乳腺癌细胞凋亡实验研究[J]. 中华核医学杂志, 2006, 26(3): 145-148 |
| |
| 作者姓名： | 熊青峰 陈跃 何菱 黄占文 李举联 邓存良 |
| |
| 作者单位： | 1. 646000 泸州医学院附属医院核医学科
2. 四川大学华西药学院 |
| |
| 基金项目： | 四川省教育厅自然科学研究基金资助项目（2004B005） |
| |
| 摘 要： | 目的研究不同放射性浓度^188Re-DTPA-脱氧葡萄糖（DG）对人MCF-7乳腺癌细胞凋亡的影响及其作用机制.方法用流式细胞仪检测^188Re-DTPA-DG处理后MCF-7乳腺癌细胞的凋亡.将人MCF-7乳腺癌细胞分成3组：^188Re-DTPA-DG实验组、^188ReO-4对照组和生理盐水组.其中前2组分3个不同放射性浓度组：37、55.5、74kBq/ml.结果^188Re-DTPA-DG、^188ReO-4均能导致肿瘤细胞发生凋亡,实验组与^188ReO-4对照组及生理盐水组在不同放射性浓度下差异均有显著性（P＜0.01）,随着放射性浓度增加,凋亡程度增大;^188Re-DTPA-DG较^188ReO-4更易诱导凋亡发生.结论^188Re-DTPA-DG能导致肿瘤细胞凋亡,并且与放射性浓度相关;其机制可能是辐射导致DNA严重损伤,使细胞周期发生阻滞.
|
| 关 键 词： | 乳腺肿瘤 肿瘤细胞 培养的 细胞凋亡 铼 脱氧葡萄糖 DTPA |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-03-13 |
The apoptosis by 188Re-DTPA-DG in breast cancer cells (MCF-7) |
| |
| XIONG Qing-feng CHEN Yu HE Ling. The apoptosis by 188Re-DTPA-DG in breast cancer cells (MCF-7)[J]. Chinese Journal of Nuclear Medicine, 2006, 26(3): 145-148 |
| |
| Authors: | XIONG Qing-feng CHEN Yu HE Ling |
| |
| Affiliation: | Department of Nuclear Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China |
| |
| Abstract: | Objective To evaluate the apoptosis by ~(188)Re-DTPA-deoxyglucose(DG)in breast cancer cells(MCF-7)and its mechanism.Methods Flow cytometry(FCM)was used to analyze apoptosis of breast cancer cells(MCF-7)with different radioactive concentration(37,55.5,74 kBq/ml)of ~(188)Re- DTPA-DG.Results Both ~(188)Re-DTPA-DG and ~(188)ReO_4~- could cause the apoptosis of cancer cells;there was significant difference among ~(188)Re-DTPA-DG,~(188)ReO_4~- and saline groups with different concentration. Apoptosis raised greatly as the concentration increased.Also,~(188)Re-DTPA-DG induced apoptosis much more than ~(188)ReO_4~-.Conclusions ~(188)Re-DTPA-DG can induce apoptosis,and is correlated with its radio- active concentration.The mechanism may be that ~(188)Re-DTPA-DG leads to the blockage of cell cycle in cancer cells. |
| |
| Keywords: | Breast neoplasms Tumor cells, cultured Apoptosis Rhenium Deoxyglucose DTPA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
