SAA 对鼻咽癌 CNE2细胞生物学行为的影响

目的：观察血清淀粉样蛋白 A（SAA）的超表达和抑制表达对鼻咽癌 CNE2细胞生物学行为的影响。方法体外培养 SAA 高表达的 pcDNA3．1（＋）-SAA-CNE2细胞系和干扰 SAA 表达的 pGPU6／GFP／Neo-SAA-CNE2细胞系，两种细胞由课题组前期重组的 SAA 高表达 pcDNA3．1（＋）-SAA 质粒及抑制 SAA 表达的 pGPU6／GFP／Neo-SAA 质粒分别转染构建。流式细胞仪分析以上两种细胞的细胞周期。平皿克隆实验检测细胞的增殖状况。结果流式细胞仪检测细胞周期显示 SAA 表达的增多可促进 CNE2细胞分裂。平皿克隆实验显示 SAA 可使 CEN2细胞的增殖能力增强。结论 SAA 具有促进人鼻咽癌 CNE2细胞增殖和体外迁移浸润的作用。

Effects of SAA protein on biological behavior of nasopharyngeal carcinoma CNE2 cells

Objective To observe the effects of over expression and inhibition expression of SAA protein on biological behavior of nasopharyngeal carcinoma CNE2 cells.Methods pcDNA3.1 (+)-SAA-CNE2 cell lines of high expression and pGPU6/GFP/Neo-SAA-CNE2 cell lines of interference expression of SAA protein in vitro.These two cells constructed by transfection of pcD-NA3.1(+)-SAA plasmid of SAA high expression and pGPU6/GFP/Neo-SAA plasmids of SAA inhibition expression respectively, plasmids of which were previously successfully reconstructed by the research group.Cell cycle of these two cells was analyzed by flow cytometry with PI staining.The ability of cell proliferation was inspected by plate cloning-forming test.Results Flow cytome-try showed that with the increase of expression of SAA protein,it had effect on promoting CNE2 cell division.Plate cloning-forming test showed that SAA protein can improve proliferation of the CNE2 cells.Conclusion SAA protein has the effect on promoting proliferation of human nasopharyngeal carcinoma CEN2 cell and migration in vitro.