| 柘树黄酮体内外抗肿瘤作用研究 |
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| 引用本文： | 张志,吴海健,皮恩浩,张填,袁文越,侯爱君,刘全海. 柘树黄酮体内外抗肿瘤作用研究[J]. 世界临床药物, 2009, 30(10): 601-605 |
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| 作者姓名： | 张志 吴海健 皮恩浩 张填 袁文越 侯爱君 刘全海 |
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| 作者单位： | 上海医药工业研究院药理室,上海,200437;复旦大学药学院,上海,201203;上海交通大学药学院,上海,200240 |
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| 基金项目： | 重大新药创制科技重大专项资助,重大新药创制科技重大专项候选药物资助,上海市科委中药现代化专项资助 |
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| 摘 要： | 目的评价柘树黄酮的抗肿瘤活性。方法采用甲基噻唑基四唑比色法体外检测柘树黄酮对15种肿瘤细胞株的生长抑制作用;建立B16黑色素瘤小鼠肿瘤模型和SKOV-3卵巢癌裸小鼠移植瘤模型,评价柘树黄酮的体内抗肿瘤活性：建立S180肉瘤小鼠肿瘤模型,考察柘树黄酮与环磷酰胺、顺铂及5-氟尿嘧啶等化疗药物的协同作用。结果柘树黄酮对人胃癌细胞（BGC-823）株、人肺癌细胞（A549）株及小鼠淋巴细胞白血病细胞（L1210）株较敏感,半数抑制浓度（IC50）分别为611、12．20和12．73ug／mL,对其他12种细胞株的细胞毒作用较小。体内试验表明,柘树黄酮能有效抑制B16黑色素瘤（250mg／kg时抑瘤率为50．54％）,并对SKOV-3卵巢癌裸小鼠移植肿瘤的生长有很好的抑制作用（250mg／kg时抑瘤率为46．38％）;柘树黄酮与化疗药物有明显的协同作用。结论体内体外试验均表明柘树黄酮有较好的抗肿瘤作用,并且与环磷酰胺、顺铂及5-氟尿嘧啶等化疗药物具有协同效应。
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| 关 键 词： | 柘树 黄酮 抗肿瘤作用 协同作用 |
Anti-tumor effects of flavonoids from Cudrania tricuspidata in vivo and in vitro |
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| ZHANG Zhi,WU Hai-jian,PI En-hao,ZHANG Zhen,YUAN Wen-yue,HOU Ai-jun,LIU Quan-hai. Anti-tumor effects of flavonoids from Cudrania tricuspidata in vivo and in vitro[J]. WORLD CLINICAL DRUGS, 2009, 30(10): 601-605 |
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| Authors: | ZHANG Zhi WU Hai-jian PI En-hao ZHANG Zhen YUAN Wen-yue HOU Ai-jun LIU Quan-hai |
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| Affiliation: | ZHANG Zhi~1,WU Hai-jian~2,PI En-hao~2,ZHANG Zhen~1,YUAN Wen-yue~3,HOU Ai-jun~(2*),LIU Quan-hai~(1*) (1.Department of Pharmacology,Shanghai Institute of Pharmaceutical Industry,Shanghai 200437,2.School of Pharmacy,Fudan University,Shanghai 201203,3.School of Pharmacy,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Objective To evaluate the anti-tumor effect of flavonoids from Cudrania tricuspidata(Carr.) Bur.in vivo and in vitro.Methods Cell proliferation was assayed by using methyl thiazolyl tetrazolium method to explore the cytotoxicity of flavonoids from Cudrania tricuspidata in vitro.B16 melanoma was induced in C57BL/ 6 mice and SKOV-3 tumor was induced in nude mice.Synergism of flavonoids from Cudrania tricuspidata with chemotherapeutic medicines in S180 sarcoma was investigated by combination with cyclophospham... |
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| Keywords: | Cudrania tricuspidata flavonoids anti-tumor effect synergism |
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