| 黄芪注射液对SPC-A1肺肿瘤细胞株细胞增殖活力和细胞凋亡影响的实验研究 |
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| 引用本文： | 盖磊,侯爱画.黄芪注射液对SPC-A1肺肿瘤细胞株细胞增殖活力和细胞凋亡影响的实验研究[J].滨州医学院学报,2014,0(4):248-250. |
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| 作者姓名： | 盖磊 侯爱画 |
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| 作者单位： | 盖磊 (滨州医学院中西医结合学院 烟台 264003 博兴县中医医院)； 侯爱画 (烟台市中医医院)； |
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| 摘 要： | 目的：观察黄芪注射液对人肺肿瘤细胞株SPC-A1细胞增殖活力和细胞凋亡影响。方法以SPC-A1肿瘤细胞株为研究材料，分别用终浓度为0、4、8、16、32μl/ml黄芪注射液刺激细胞株，分别用CCK8 kit法检测细胞活力和用流式细胞仪PI染色检测细胞凋亡。结果高浓度的黄芪注射液对SPC-A1的增殖有促进作用，对其凋亡无显著性影响。结论高浓度黄芪注射液促进SPC-A1增殖。
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| 关 键 词： | 黄芪注射液 肺癌 细胞增殖 细胞凋亡 |
The experimental research about the effect of Astragalus membranaceus injection on cell proliferation activity and cell apoptosis of SPC-A1 lung cancer cell line |
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| Institution: | GAI Lei, HOU Aihua (1 Combined Traditional Chinese and Western Medicine College,Binzhou Medical University, Yantai 264003; 2 Boxing Traditional Chinese Medicine Hospital;3 Yantai Traditional Chinese Medicine Hospital) |
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| Abstract: | Objective To observe the effect of astragalus membranaceus injection on cell proliferation activity and cell apopto-sis of SPC-A1 lung cancer cell line .Methods SPC-A1 lung cancer cell line was used as research material ,which was stimulated respectively by astragalus membranaceus injection with 0 ,4 ,8 ,16 ,32μl/ml of final concentration .CCK8 kit method was used to detect cell proliferation activity of SPC-A1 lung cancer cell line ,and flow cytometry with PI staining method to detect cell apop-tosis .Results Astragalus membranaceus injection with high concentration had auxo-action to SPC-A1 cell proliferation ,and had no effect on SPC-A1cell apoptosis .Conclusions Astragalus membranaceus injection with high concentration can promote SPC-A1 cell proliferation . |
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| Keywords: | Astragalus membranaceus injection lung cancer cell proliferation cell apoptosis |
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