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| BMP调控成骨细胞生物活性的实验研究 | |
| 引用本文： | 张学敏,马文辉,时述山,李亚非.BMP调控成骨细胞生物活性的实验研究[J].河北医药,2009,31(6):649-651. |
| 作者姓名： | 张学敏 马文辉 时述山 李亚非 |
| 作者单位： | 1. 河北国防建医院,石家庄市,050018 2. 白求恩国际和平医院 3. 北京军区总医院 |
| 摘 要： | 目的 探讨骨形态发生蛋白（bone morphogenetic protein，BMP）对培养的正常人成骨细胞主要生物学行为的影响。方法分别以0．02、0．1、1μg/m3种浓度的BMP作用于成骨细胞，通过MTY法了解BMP的效应。以0．1μg/ml浓度的BMP作用于成骨细胞，测定BMP对成骨细胞碱性磷酸酶（alkaline phospbatase，ALP）活性及骨钙素（bone gla protein，BGP）合成等主要生物学特性的影响。结果BMP作用于成骨细胞，能促进ALP活性及BGP的合成。结论BMP能促进成骨细胞活性，并且其促进成骨增殖的效能具有浓度依赖性。 |
| 关 键 词： | 骨形态发生蛋白 成骨细胞 生物学活性 |
Study of the effect of BMP on the biological activity of osteoblast in healthy people in vitro |
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| ZHANG Xuemin,MA Wenhui,SHI Shushan,et al..Study of the effect of BMP on the biological activity of osteoblast in healthy people in vitro[J].Hebei Medical Journal,2009,31(6):649-651. | |
| Authors: | ZHANG Xuemin MA Wenhui SHI Shushan |
| Institution: | ZHANG Xuemin,MA Wenhui,SHI Shushan,et al.Hebei National Defence Construction Hospital,Shijiazhuang 050018,China |
| Abstract: | Objective To investigate the effect of bone morphogenetic protein （BMP） on the biological activity of osteoblast of healthy people in vitro. Methods The osteoblast was stimulated with BMP at the concentrations of 0.02, 0.1 μg/ml respectively, and the effects of BMP at different concentrations were detected by MTT. The osteoblast was stimulated with BMP at the concentration of 0.1 μg/ml, and the levels of alkaline phosphatase （ALP） and bone gla protein （BGP） of osteoblast were measured. Results BMP could promote osteoblast to synthesize more ALP and BGP. Conclusion BMP can increase the activity of osteoblast, which is concentration-dependent. |
| Keywords: | bone morphogenetic protein osteoblast biological activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |