MK-801对新生大鼠脑外伤后神经元凋亡的影响

目的：探讨N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801对新生大鼠创伤性脑外伤(traumatic brain injury,TBI)后神经元凋亡的影响。方法：建立新生7 d大鼠顶叶皮质挫伤模型,在TBI前30 min、TBI后即刻、TBI后30 min分别给予腹腔注射MK-8011 mg/kg,在TBI后24 h取脑,连续切片,行H-E染色和Caspase-3免疫组化染色,检测脑神经元细胞的损伤和凋亡。结果：MK-801三组不同时间用药组与TBI组相比,在创伤同侧的扣带皮质、顶叶皮质和丘脑神经元凋亡细胞数减少,有显著性差异。其中TBI后即刻用MK-801治疗效果最好。结论：MK-80l能明显减少TBI后神经元的凋亡。

Effect of MK-801 on neuronal apoptosis after tratumatic brain injury in neonatal rats

Objective:To study the effect of MK-801,the antagonist of NMDA receptor,on neuronal apoptosis after traumatic brain injury (TBI) in neonatal rats.Methods:Seven-day-old rat pups were subjected to concussion trauma on the parietal cortex and were injected with 1 mg/kg MK-801 30 minutes before TBI,immediately after TBI and 30 minutes after TBI.The pups were killed 24 h after injection.The neuronal apoptosis of the brain was investigated by both HE and Caspase-3 immunostaining.Results:Compared with TBI group,the number of ap- optosis cells in MK-801-treated group was significantly decreased in the ipsilateral cingulate,parietal cortex and thalamus.The outcome was best when MK-801 was used immediately after head trauma.Conclusion:MK-801 can reduee apoptosis of cells in the immature brain after TBI.