Association of parental hyperhomocysteinemia and C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism with recurrent pregnancy loss |
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| Authors: | Vinukonda Govindaiah Shaik Mohammad Naushad Krishnamurthy Prabhakara Prasad Chintakindi Krishna Akella Radha Rama Devi |
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| Affiliation: | 1. Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;2. Department of Obstetrics and Gynecology, 1st Faculty of Medicine and General University Hospital, Prague, Czech Republic;3. Department of Preventive Cardiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic;4. Department of Surgery, Penn Transplant Institute, Perelman School of Medicine, University of PA, Philadelphia, USA;2. Department of Public Health, Hokkaido University Graduate School of Medicine, Kita 15, Nishi 7, Kita-ku, Sapporo, 060-8638 Japan;1. Department of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo 105-8461, Japan;2. Department of Maternal-Fetal Biology, National Center Institute for Child Health and Development, Tokyo 157-8535, Japan;3. Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa 216-8511, Japan;4. Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa 216-8511, Japan |
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| Abstract: | ObjectivesTo investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL).Design and methodsA case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to assess total plasma homocysteine, C677T MTHFR polymorphism and DNA damage respectively. Student t-test, ANOVA and Fisher exact test were used for statistical analysis.ResultsMaternal [mean: 11.6 ± 5.0 versus 8.6 ± 4.2 μmol/L, odds ratio (OR): 4.48] and paternal [mean: 19.6 ± 9.5 versus 14.2 ± 7.4 μmol/L, OR: 6.92] hyperhomocysteinemia, paternal age [OR: 1.16], paternal MTHFR 677T allele [OR: 2.30] and DNA damage were found to increase the risk for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele.ConclusionsParental hyperhomocysteinemia, paternal age, paternal C677T MTHFR polymorphism and DNA damage are risk factors for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele. |
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