实时荧光依赖解旋酶恒温扩增技术和实时荧光RT-PCR在手足口病中的应用价值

目的 研究实时荧光依赖解旋酶恒温扩增技术和实时荧光RT-PCR在手足口病中的应用价值.方法 回顾性分析106例疑似手足口病患儿的临床资料.所有患儿均进行实时荧光RT-PCR检测、实时荧光依赖解旋酶恒温扩增技术检测,以临床表现、实验室检查、血清和病原学的综合诊断结果为临床诊断金标准,比较不同检测方法的诊断结果及效能.结果...

Application value of real-time fluorescence dependent helicase isothermal amplification and real-time fluorescent RT-PCR in the hand-foot-and-mouth disease

Objective To study the application value of real-time fluorescence dependent helicase isothermal amplification and real-time fluorescent RT-PCR in the hand-foot-and-mouth disease.Methods The clinical data of 106 children with suspected hand-foot-and-mouth disease were retrospectively analyzed.All children were detected by real-time fluorescent RT-PCR and real-time fluorescence dependent helicase isothermal amplification.The comprehensive diagnosis results of clinical manifestations,laboratory examination,serum and etiology were taken as the clinical diagnostic gold criteria.The diagnostic results and efficacy of different detection methods were compared.Results The window period of EV universal,EV71 and CoxA16 and average time detected by real-time fluorescence dependent helicase isothermal amplification were shorter than those by real-time fluorescence RT-PCR,the diagnostic efficiency were better than those by real-time fluorescence RT-PCR,and the positive comprehensive diagnostic rate was higher than that by real-time fluorescence RT-PCR(P<0.05).Conclusion The real-time fluorescence dependent helicase isothermal amplification has the advantages of fast,high efficiency and high diagnostic efficiency in the detection of hand-foot-and-mouth disease,and which has high clinical promotion and application value.