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Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring
Authors:Nichola C. Garbett  Chongkham S. Mekmaysy  C. William Helm  A. Bennett Jenson  Jonathan B. Chaires
Affiliation:1. James Graham Brown Cancer Center, University of Louisville, 529 S. Jackson Street, Room 428, Louisville, KY 40202, USA;2. Division of Gynecologic Oncology, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA;1. Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China;2. Department of Cardiology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;3. Analytical and Testing Center, Jinan University, Guangzhou 510632, China;4. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau 999078, China;5. Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China;6. Department of Respiratory Medicine, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;1. Department of Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;2. Department of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan;3. Genome Science Division, RCAST, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan;1. Department of Physics and Astronomy, Tufts University, Medford, MA 02155, USA;2. Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA;3. Institute for Physics, University of Rostock, Rostock, Germany;1. Materials Science and Engineering, Yeungnam University, Gyeongsan 712-749, Gyeongbuk, Republic of Korea;2. Advanced Ceramics Group, National Institute for Materials Science, Tsukuba 305-0047, Ibaraki, Japan;1. State Key Laboratory of Fine Chemicals, Institute of Artificial Photosynthesis, DUT-KTH Joint Education and Research Centre on Molecular Devices, Institute for Energy Science and Technology, Dalian University of Technology, Dalian 116024, Liaoning, China;2. Department of Theoretical Chemistry and Biology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm 10691, Sweden;3. Department of Chemistry and Shenzhen Grubbs Institute, Southern University of Science and Technology (SUSTech), Shenzhen 518055, Guangdong, China;4. Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm 10044, Sweden
Abstract:
Differential scanning calorimetry (DSC) provides a useful method to study the unfractionated plasma proteome. Plasma from healthy individuals yields a reproducible signature thermogram which results from the weighted sum of the thermal denaturation of the most abundant plasma proteins. Further investigation of the thermogram for healthy individuals showed it to be sensitive to ethnicity and gender. DSC analysis of plasma from diseased individuals revealed significant changes in the thermogram which are suggested to result not from changes in the concentration of the major plasma proteins but from interactions of small molecules or peptides with these proteins. Closer examination of the diseased thermograms showed a thermogram characteristic of each disease. For cervical cancer, the DSC method yields a progressively shifted thermogram as the disease advances from pre-invasive conditions to late stage cancer. Our application of the DSC method has provided a potential tool for the early diagnosis, monitoring and screening of cancer patients.
Keywords:
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