|
|||||
|
|
| 青蒿琥酯诱导垂体泌乳素瘤细胞凋亡并下调泌乳素蛋白表达 | |
| 引用本文： | 陈春华,温保全,肖伟伟,雷霓,朱永红. 青蒿琥酯诱导垂体泌乳素瘤细胞凋亡并下调泌乳素蛋白表达[J]. 解剖学研究, 2011, 33(6): 422-426 |
| 作者姓名： | 陈春华 温保全 肖伟伟 雷霓 朱永红 |
| 作者单位： | 1. 中山大学中山医学院组织学与胚胎学教研室,广东 广州,510080 2. 顺德中医院神经外科,广东 佛山,528300 |
| 基金项目： | 广东省自然科学基金,广东省社会发展科技计划项目,广东省中医药计划科研基金,高等学校博士学点专项科研基金 |
| 摘 要： | 目的 初步研究青蒿琥酯( artesunate,ART)对泌乳素瘤细胞及其泌乳素(prolactin,PRL)蛋白表达的作用效果.方法 MTT观察青蒿琥酯作用细胞后,细胞增殖情况,并计算药物的半数抑制浓度(IC50),Hoechst33342染色观察细胞凋亡小体,TUNEL计算细胞凋亡率,Western blot和RT... |
| 关 键 词： | 垂体腺瘤 青蒿琥酯 泌乳素瘤细胞系 凋亡 泌乳素 |
Artesunate induces apoptosis of pituitary adenoma cell line and reduces the level of PRL |
|
| CHEN Chun-hua , WEN Bao-quan , XIAO Wei-wei , LEI Ni , ZHU Yong-hong. Artesunate induces apoptosis of pituitary adenoma cell line and reduces the level of PRL[J]. Anatomy Research, 2011, 33(6): 422-426 | |
| Authors: | CHEN Chun-hua WEN Bao-quan XIAO Wei-wei LEI Ni ZHU Yong-hong |
| Abstract: | Objective To observe the effect of artesunate(ART) on prolactinoma cells.Methods The rat pituitary adenoma cell line(MMQ) was chosed as in vitro cell model.MTT method was used to investigate the growth of MMQ cell after treated by ART.Hoechst33342 and TUNEL assaies used to observe apoptotic body and apoptotic rate.The levels of PRL and PRLmRNA after ART treatment were investigated by Western blot and RT-PCR.Results ART inhibited the growth of MMQ cell.Contrast with the control group,Hoechst 33342 assay showed that there were many apoptotic bodies.ART reduced the expressions of PRL and PRLmRNA,which were observed by Western blot and RT-PCR.Conclusion ART can induce apoptosis of MMQ and reduce the secretion of PRL. |
| Keywords: | Pituitary adenoma Artesunate Prolactinoma cell line Apoptosis Prolactin |
| 本文献已被 万方数据 等数据库收录！ | |