高效液相色谱法测定嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱含量

目的建立测定嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱含量的高效液相色谱法。方法色谱柱为Symmetry C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水-氯仿-冰醋酸-三乙胺(50∶50∶2∶1∶0.6),柱温为25℃,流速为1.0 mL/min,检测波长为254 nm。结果乌头碱、中乌头碱和次乌头碱的质量浓度均在9.0～45.0μg/mL范围内与峰面积线性关系良好,回归方程分别为A1=2.125×106C+1.252×104,A2=2.143×106C+1.422×104,A3=1.968×106C-2.152×104,相关系数r分别为0.999 6,0.999 8,0.999 8,平均回收率分别为100.40%,99.61%,100.15%,RSD分别为0.72%,0.86%,0.89%。结论所用方法简便、快速、准确,适用于嘎日迪五味丸中乌头碱、中乌头碱和次乌头碱的含量测定。

Content Determination of Aconitine,Mesaconitine and Hypaconitine in Garidi-5 Pills by HPLC

Objective To establish HPLC method for determining the content of aconitine,mesaconitine and hypaconitine in Garidi-5 Pills.Methods The chromatographic column was Symmetry C18（150 mm×4.6 mm,5 μm）,the mobile phase was methol-water-chloroform-glacial acetic acid-thriethylamine（50：50：2：1：0.6）,the column temperature was 25 ℃,the flow rate of 1.0 mL/min,the UV detection wavelength was 254 nm.Results The linear range of concentration of three compounds was 9.0-45.0 μg/mL.Regression equations were A1=2.125×106C＋1.252×104,A2=2.143×106C＋1.422×104,A3=1.968×106C-2.152×104,r were 0.999 6,0.999 8,0.999 9,respectively.The average recoveries and the relative standard deviation were 100.40%,99.61%,100.15%and 0.72%,0.86%,0.89%,respectively.Conclusion The method is simple,fast and accurate.It is suitable for content determination of aconitine,mesaconitine and hypaconitine in Garidi-5 Pills.