大鼠清醒状态下尾静脉注射的快速操作技巧与体会

目的介绍大鼠清醒状态下尾静脉注射的快速操作技巧与体会,提高操作成功率。方法采用自制大鼠束缚衣结合操作者轻按的方法固定大鼠,一人辅助固定,一人穿刺,一人注药,在注射过程中尽量减少对鼠尾的损伤和对大鼠产生的应激,严格规定进针点和穿刺次数,限制一次性最大给药剂量和注射速度,并采取将进针与注射药液的部位分开等方法避免药物污的染和浪费。结果本法操作快速、可靠、稳定、重复性好、动物配合度高。结论本法避免了药物的浪费和污染,对于药物昂贵的尾静脉注射实验此做法尤为推荐。

A quick operating technique and experience of tail vein injection in conscious rats

Objective To introduce the operation skills of tail vein injection in conscious rats, and improve the success rate of ingection. Methods The rat was fixed by the operator with a self-made binding clothes, one person assists to fix the rat tail, one person performs puncture, and one person performs injection. During the injection process, we should minimize the injury to the rat tail and the stress caused by operation, strictly limit the needle point, the number of puncture, the maximum dose and injection speed, and to make the needle position away from the injection site to avoid contamination and waste of the drug solution. Results The operation method was successfully established and it was fast, stable, with good repeatability and high degree of coordination. Conclusion This operation is rapid, reliable and stable, worthy of recommendation, especially for the intravenous injection of expensive drugs.