提高犬交感神经相关房颤模型建模成功率的新方法

目的对传统实验过程中犬的星状神经节暴露的方法进行改良,建立一种新的暴露犬星状神经节方法,提高交感神经相关房颤模型建模的成功率。方法成年犬28只,随机分为传统组和改良组,每组14只,分别使用传统和改良后的方法分离暴露星状神经节。28只犬均建立交感神经相关房颤模型。术中记录犬生命体征变化和术中生存率,记录刺激星状神经节所需电压,记录刺激前、后心脏电生理的变化。免疫组化检测神经递质释放情况。结果改良组术中存活率较传统组术中存活率显著升高,P0.05。改良组手术时间较传统组明显缩短,P0.05。刺激改良组神经达到阈值所需电压较传统组更小,P0.05。结论改良后的手术方法能够有效的降低犬在手术过程中的死亡率,明显缩短手术时间,减少术中出血量,星状神经节结构更完整,持续刺激过程中所需电压更稳定,更适合用于建立交感神经相关房颤模型。

A new method to improve the success rate of establishing a canine model of atrial fibrillation induced by sympathetic stimulation

Objective To develop a new method to expose the stellate ganglion to increase the success rate of establishing a dog model of atrial fibrillation indinced by sympathetic stimulation. Methods A total of 28 adult dogs were randomly divided into traditional group and improvement group, 14 dogs in each group. The stellate ganglions were separated by the two different methods, respectively, to establish a sympathetic stimulation induced atrial fibrillation model in all the dogs. Changes of vital signs, survival rate of the dogs and the voltage required to stimulate the stellate ganglion were recorded intraoperatively. Changes of cardiac electrophysiology were recorded before and after electric stimulation. The levels of released neurotransmitters were detected by immunohistochemistry. Results The survival rate of the improvement group was 100% (14/14), significantly higher than the 64.3% (9/14) of the traditional group (P<0.05). The operation time of the improvement group was 122.71±3.62 min, significantly shorter than the 269.44±8.79 min of the traditional group (P<0.05). The threshold voltage of the improvement group was significantly lower than that of the traditional group (P<0.05). Conclusions Our modified surgical procedure can effectively reduce the mortality of dogs, significantly shorten the operation time, and reduce the intraoperative blood loss, keeping a more intact stellate ganglion, and maintains a more stable voltage of electric stimulation, Therefore, it is a new method more suitable for establishment of a sympathetic stimulation induced atrial fibrillation model in dogs.