自发性肝硬化比格犬肝脏病理形态学研究

目的研究自发性肝硬化比格犬肝脏的病理形态学改变和血清生化改变,以建立适用于GLP的实验动物背景资料。方法下肢静脉采血制备血清,全自动生化分析仪测ALT、AST、TP、ALB、ALP、TBIL、TC、TG、GGT等肝功能指标,比较患病动物与同期空白对照组动物上述指标的差异;对比格犬肝脏进行组织病理学检查,光学显微镜下描述其病理形态学特点。结果与同期空白对照组动物相比,患病动物ALT、AST、ALP、TBIL、GGT显著升高,ALB显著降低;与正常组织相比,肝硬化的肝细胞呈结节状再生、排列紊乱,形成数量不等的假小叶,假小叶周围被增生的胶原纤维所包裹。结论需加强比格犬自发病变的病理监测,为药物安全性评价提供实验动物的背景资料。

Study on pathomorphological Changes of Iiver in Beagle dog with Spontaneous hepatocirrhosis

Objective To study the liver pathomorphological and serum biochemical changes in Beagle dog with spontaneous hepatocirrhosis and establish the backgroud information of experimental animals for GLP. Methods The ALT,AST,TP,ALB,ALP,TBIL,TC,TG and GGT were detected using an automatic biochemical analyzer,and compared the differences of above index between blank control and diseased animal.The histological changes of the liver were observed by optical microscopy. Results Compared with the blank control,the ALT,AST,ALP,TBIL and GGT of diseased dogs were increased significantly,and the ALB decreased significantly. Compared with normal dogs, the liver cells had nodular regeneration, arranged irregularly and pseudolobule formation.The pseudolobules were packaged with collagen fibers.Conclusion It is suggested that spontaneous lesions in Beagle dogs should be monitored so as to provide appropriate experimental animal histopathologicalbackgroud information for drug safety evaluation.