c-myc和k-ras基因多态性与辐射诱发小鼠胸腺淋巴瘤的关联性分析

目的：探讨c-myc和k-ras基因多态性与辐射诱发小鼠胸腺淋巴瘤的关系。方法：采用 ⁶⁰Co（剂量率0.382 Gy•min^-1，总剂量7 Gy）分别照射C57BL/6J和BALB/c小鼠建立小鼠胸腺淋巴瘤模型，6个月后处死小鼠，取胸腺组织，用Promega DNA纯化试剂盒提取胸腺淋巴瘤细胞基因组DNA，以聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法对c-myc基因rs51048361（C/T）、k-ras基因rs30221756（A/G）和rs30161609（T/C）单核苷酸多态性进行检测。结果：⁶⁰Co照射引起2种小鼠的胸腺瘤发生率不同，对应的 rs51048361位点基因型分别为C/C和T/T，rs30221756位点基因型分别为A/A和G/G，rs30161609位点基因型分别为T/T和C/C。结论：辐射敏感性不同的C57BL/6J和BALB/c 小鼠 ⁶⁰Co照射后rs51048361、rs30221756和rs30161609　位点基因型不同，推测c-myc和k-ras基因多态性与辐射诱发胸腺淋巴瘤有关联。

Analysis on relevance between polymorphism of c-myc and k-ras gene and thymic lymphomas of mice induced by ionizing radiation

Objective To investigate the relation between polymorphism of c-myc and k-ras gene and thymic lymphomas of mice induced by ionizing radiation. Methods The thymic lymphomas models of mice were made by 60Co(dose rate:0.382 Gy·min-1, total dose: 7 Gy)exposuring to C57BL/6J and BALB/c,6 months later,the mice were killed and thymus tissues were taken, genome DNA of thymic lymphomas cell was extracted by Promega DNA purification kit, PCR-restriction fragment length polymorphism (RFLP) was used to examine the single nucleotide polymorphism(SNP)of rs51048361(C/T)of c-myc gene,rs30221756(A/G)and rs30161609(T/C)of k-ras gene. Results The thymic lymphomas incidence induced by 60Co exposuring in two kinds of mice was different,the genotypes of rs51048361 site were C/C and T/T,the genotypes of rs30221756 site were A/A and G/G,the genotypes of rs30161609 site were T/T and C/C.Conclusion After 60Co exposuring,the genotypes of rs51048361,rs30221756 and rs30161609 in C57BL/6J and BALB/c with different radiosusceptibility are different,so it is inferred that there is relevance between the polymorphism of c-myc and k-ras gene and thymic lymphomas induced by ionizing radiation.