烟台市铜绿假单胞菌质粒、转座子、整合子遗传标记研究

目的了解烟台市铜绿假单胞菌（Pseudomonas aeruginosa，PAE）质粒、转座子、Ⅰ类整合子遗传标记存在状况。方法采用K-B法测定临床分离的30株铜绿假单胞菌对16种抗菌药物的敏感性，采用聚合酶链反应法（PCR）对其进行质粒遗传标记（traA、traF）、转座子遗传标记（tnpA、tnpU、merA）、Ⅰ类整合子遗传标记（intⅠ）检测。结果30株铜绿假单胞菌对16种抗菌药物均有不同程度的耐药。30株PAE菌merA阳性14株，阳性率46．7％；intⅠ阳性14株，阳性率46．7％。其中merA与intⅠ基因同时阳性11株，单独merA基因阳性3株，单独intⅠ基因阳性3株；共有17株检出merA和／或intⅠ基因。其它均为阴性。结论临床分离的铜绿假单胞菌转座子遗传标记和Ⅰ类整合子遗传标记检出率较高。本组PAE菌没有检出F质粒和RP4质粒的遗传标记。

Study of Genetic Markers of Plasmid,Integrons and Transpasons in Pseudomonas aeruginosa in Yantai

Objective To identify the presence of drug resistance genes in plasmid, integrons and transpasons of pseudomonas aeruginosa in Yantai. Methods The susceptibility to 16 antibiotics was tested in 30 clinically isolated pseudomonas aeruginosa by paper diffusing method, PCR method was used to amplify the following genes from 30 strains of pseudomonas aeruginosa., genetic markers of plasmid（traA, traF）, integrons （tnpA、 tnpU, merA） and Ⅰ transposons （int Ⅰ ）. Results Different degreeof drug resistance were showed in 30 strains of pseudomonas aeruginosa. The positive rate of drug resistance gene of merA was 46.7% （14/30）, the positive rate of int Ⅰ was 46.7% （14/30）. Among them 11 strains contain an array of drug resistance genes as well as merA and integron Ⅰ, 3 strains contain drug resistance genes of merA and other 3 strains contain drug resistance genes of integron Ⅰ. The total positive rate of merA and/or int Ⅰ gene was 56.7% （17/30）. The rest kinds of genes were all tested negative. Conclusion There was higher positive rate of integrons and transpasons gene in pseudomonas aeruginosa isolated from the inpatients in Yantai.No genetic markers of plasmid of A or F was tested.