电针足阳明经穴对家兔胃粘膜损伤细胞保护作用的研究

本实验采用无水乙醇灌胃造成胃粘膜损伤后 ,分别电针足阳明经“四白”、“梁门”、“足三里”三个不同段代表穴 ,及“足三里”外 2cm的对照点 ,观察其对胃粘膜损伤后的细胞保护作用 ,以证实足阳明经与胃的相关性。结果发现 ,分别电针“四白”、“梁门”、“足三里”7日后 ,均能使胃粘膜损伤指数显著降低。电针“足三里”后 ,胃液及胃粘膜PGE2 与其他各组比较均显著增高 (P <0 0 5或 0 0 1 ) ,血清NO与模型组、电针“足三里”外 2cm组比较亦见明显增高 (P <0 0 5) ;电针“四白”、“梁门”、“足三里”以及空白组胃粘膜EGF与未经电针的模型组比较均有显著差异 (P <0 0 5或0 0 1 )。提示 ,电针家兔足阳明经不同节段的腧穴均对胃粘膜损伤细胞有保护作用 ,其中以“足三里”最满意 ,说明足阳明经与胃具有相关性 ,与此同时 ,同一经脉的穴位对相关脏腑的作用亦有着相对的特异性

Effect of Electroacupuncture of Acupoints of Stomach Meridian on Gastric Mucosal Tissues in Alcohol-induced Gastromucosal Lesion Rabbits

Objective: To observe the effect of electroacupuncture (EA) of "Sibai" (ST 2), "Liangmen" (ST 21) and "Zusanli" (ST 36) on gastric mucosal tissues in alcohol induced gastromucosal lesion rabbits for analyzing the relationship between Foot Yangming Meridian and stomach. Methods: 48 rabbits were randomly and evenly divided into "Sibai" (ST 2), "Liangmen" (ST 21), "Zusanli" (ST 36), non acupoint, model and control groups. Gastric mucosal injury model was induced by intragastric infusion of dehydrated alcohol (2.35 mL/kg). The non acupoint was located at the site about 2 cm lateral to "Zusanli" (ST 36). EA parameters were frequency of 4～20 Hz, pulse width 0.5 ms, voltage 60 V (peak peak value, no load), and stimulation duration 20 min. The treatment was given once daily, continuously for 7 days. Prostaglandin (PG) E 2, gastrin (GAS), epidermal growth factor (EGF) of plasma, gastric juice and gastric mucous tissue concentrations were detected with radioimmunoassay and serum nitric oxide (NO) level was determined by using ultraviolet spectrophotometry. Results: After EA of the above mentioned 3 acupoints, the gastric mucosal injury index decreased significantly in comparison with non acupoint group (P<0.05). PGE 2 levels in plasma, gastric juice and mucous tissues in EA of "Zusanli" group were all obviously higher than those of the rest groups (P<0.05 or 0.01); and serum NO level of "Zusanli" group was also higher than that of the rest groups, markedly higher than that of model group and non acupoint group (P<0.05). After generation of gastric mucosal injury, EGF level of gastric mucous tissues decreased significantly in comparison with control group. While gastric mucosal EGF levels in "Sibai", "Liangmen" and "Zusanli" and control groups were all obviously higher than that of model group (P<0.05 or 0.01), but no significant difference was found between non acupoint group and model group (P>0.05). Conclusion: These findings suggest that EA of different acupoints of Foot Yangming Meridian has a protective effect on gastric mucosal lesion; among those acupoints, and the effect of "Zusanli" (ST 36) is the best. It indicates that there is a close relationship between Foot Yangming Meridian and stomach.