氡及其子体对大鼠肺及其支气管的损伤作用

[目的 ]研究大鼠吸入氡及其子体后肺及支气管组织的生物学变化。 [方法 ]雄性SD大鼠吸入氡及其子体的累积剂量分别达 66、111、174工作水平月 (workinglevelmonth ,WLM )后 ,收集支气管肺灌洗液 (bronchialveolarlavageflu ids ,BALF) ,观察BALF中细胞计数和分类的变化。采用SCGE技术 ,检测BALF和肺细胞的DNA链断裂情况。同时观察大鼠气管、支气管、细支气管和肺泡的病理组织学变化。 [结果 ]大鼠吸入氡及其子体后 ,各染毒组BALF中细胞总数有增加趋势 ,但仍在正常范围之内 ,淋巴细胞比例明显减少 ,而粒细胞增多 (P <0 0 5 )。各染毒组细胞DNA链断裂的迁移长度显著增加 ,且存在剂量 效应关系。病理结果观察到 174WLM剂量组肺组织慢性炎细胞浸润明显 ,部分区域浸及肌层 ,肺泡上皮大片脱落 ,出现灶性肺气肿。 [结论 ]在本实验的染毒剂量下 ,氡及其子体可降低BALF中淋巴细胞比例 ,增加粒细胞比例 ;能引起BALF和肺细胞的DNA链断裂的损伤

The Damage Effects on the Lung in Rats Inhaled Radon and Radon Progeny

To study biological changes of the lung in rats exposed to radon and its progeny. Sprague Dawley rats were exposed to radon and its progeny with the cumulative dose up to 66,111 or 174 working level month(WLM),respectively. The numbers and proportions of cells in bronchi alveolar lavage fluids(BALF) were investigated. The DNA migration in the BALF and lung cells was detected with single cell gel eletrophoresis(SCGE). The pathological changes of lung were examined. It was shown that proportions of BALF cells decreased in the proportion of lymphocytes and increased in proportion of granulocytes ( P <0 05). The movements of DNA migration in the BALF and lung cells increased with a dose effect relationship. The pathological change of lung was characterized by chronic inflammation and focal pulmonary emphysema. [Conclusion] Radon and its progeny could decrease the proportion of lymphocytes in BALF and increase the moments of DNA migration in BALF and lung cells.