| 金葡菌及L型诱导脐静脉内皮细胞凋亡的研究 |
| |
| 引用本文： | 管俊昌 刘勇 徐飞 夏佩莹. 金葡菌及L型诱导脐静脉内皮细胞凋亡的研究[J]. 中国人兽共患病杂志, 2004, 20(11): 951-953,950 |
| |
| 作者姓名： | 管俊昌 刘勇 徐飞 夏佩莹 |
| |
| 作者单位： | 蚌埠医学院病原生物学教研室 蚌埠233003(管俊昌，刘勇，徐飞)，蚌埠医学院病原生物学教研室 蚌埠233003(夏佩莹) |
| |
| 基金项目： | 安徽省自然科学基金资助项目(No.01043706)，安徽省教育厅自然科学研究计划项目(No.2001kj172、No.2002kj206) |
| |
| 摘 要： | 目的比较金黄色葡萄球菌(金葡菌)及其L型诱导人脐静脉内皮细胞(HUVECs)凋亡的差异。方法用TUNEL法及流式细胞仪检测金葡菌及其L型感染HUVECs2、4、6、8与10h后各时间段的细胞凋亡率。结果金葡菌及其L型均能诱导HUVECs发生凋亡,与对照组比较差异有显著性意义(P<0.05或P<0.01);L型诱导细胞的凋亡率均弱于金葡菌(P<0.01)。结论金葡菌L型诱导HUVEC发生凋亡的能力虽较其原型弱,但其对胚胎发育的潜在致病性值得重视。
|
| 关 键 词： | 金葡菌L型 诱导 脐静脉 内皮细胞 细胞凋亡 |
| 文章编号： | 1002-2694(2004)11-0951-03 |
Study on the apoptosis of the human umbilical vein endothelial cells induced by Staphylococcus aureus and its L forms |
| |
| GUAN Jun chang,LIU Yong,XU Fei,XIA Pei ying. Study on the apoptosis of the human umbilical vein endothelial cells induced by Staphylococcus aureus and its L forms[J]. Chinese Journal of Zoonoses, 2004, 20(11): 951-953,950 |
| |
| Authors: | GUAN Jun chang LIU Yong XU Fei XIA Pei ying |
| |
| Abstract: | To compare the differences of the induced apoptosis of the human umbilical vein endothelial cells ( HUVEC) by Staphylococcus aureus and its L forms, rates of apoptosis in HUVEC cells at different times after infection with S.aureus or its L forms were determined by TUNEL method and flow cytometry.It was found that both S.aureus and its L forms could induce apoptosis of the HUVEC cells with significant difference in the rates of apoptosis with those of the controls. However, the rates of apoptosis induced by L forms were obviously lower than that induced by S.aueus itself.It concludes that this difference in the ability to induce cell apoptosis of HUVEC cells by S.aureus and its L forms may be related to the latent pathogenicity to the S.aueus L forms on the embryonic development. |
| |
| Keywords: | Staphylococcus aureus bacterial L forms endothelial cell apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
