镉在肝细胞内的吸收过程及其对肝细胞内钙稳态的影响

采用游离原代肝细胞培养技术，观察镉（Ｃｄ）在肝细胞内的吸收过程及其对肝细胞内钙稳态的影响。实验发现，肝细胞对Ｃｄ的吸收分为２个时期，首先是一个迅速吸收期，随后有一个缓慢吸收期；实验还发现，在Ｃｄ作用浓度为３．５６μｍｏｌ／Ｌ时，肝细胞内钙稳态并未发生明显变化；与此同时，肝细胞内ＣａＭ活性和Ｃａ（２＋）－Ｍｇ（２＋）－ＡＴＰ酶活性持续升高；结果揭示，Ｃｄ可能通过激活ＣａＭ，导致Ｃａ（２＋）－Ｍｇ（２＋）－ＡＴＰ酶活性升高，增强了生物膜的钙隔离作用，造成了细胞内游离钙离子浓度不变。

Cadmium Uptake in Hepatocyte and Effects of Cadmium to Intracellular Calcium Homestasis

Using the hepatocyte primary culture techniques,Cadmium uptake by rat hepatocytes and the effects of cadmium to intracellular calcium homestasis were observed. The result suggested that a rapid phase of cadmiun uptake by rat hepatocytes was followed by a second slower phase. As the Cadmium exposure time prolonging the calcium homestasis in hepatocyte show no markedly alteration, the activity if calmodulin and Ca -Mg -ATPase increased significantly. Those result also suggested that Cadmium can elevated the activity of Ca -Ng -ATP ase by activiting the CaM, leading the calcium homestsis in hepatocyte shows no markedly alteration.