Effect of High Molecular Weight Water-Soluble Chitosan on the Trabecular Bone and Thickness in Ovariectomized Rats |
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| Authors: | Han-Jung Chae Geun-Youn Lee Sun-Kyung Yang Do-Sung Kim Ki-Jung Yun Eun-Cheol Kim Hyung-Min Kim Soo-Wan Chae Hyung-Ryong Kim |
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| Affiliation: | a Department of Pharmacology and Institute of Cardiovascular Research, Medical School, Chonbuk National University, Jeonju, Chonbuk, South Koreab Department of Dental Pharmacology and Wonkwang Biomaterial Implant Research Institute, School of Dentistry, Wonkwang University, Iksan, Chonbuk, South Koreac Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, South Koread Department of Oral and Maxillofacial Pathology, School of Dentistry, Wonkwang University, Iksan, Chonbuk, South Koreae Department of Oriental Pharmacology, School of Oriental Medicine, Kyung Hee University, Seoul, South Korea |
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| Abstract: | High molecular weight water-soluble chitosan(WSC), having an average molecular weight of 300,000 Da and a degree of deacethylation over 90%, can be produced using a simple multi-step membrane separation process. In this study, the trabecular bone area and thickness in ovariectomized(OVX) rats decreased by almost 50% from those in sham-operated rats. WSC was evaluated for inhibition of the progress of bone loss induced by OVX rats. We measured bone histomorphometry in sham, OVX or WSC-administered OVX rats. From light microscopic analyses, a porous or erosive appearances were observed on the surface of trabecular bone of tibia in OVX rats, whereas those of the same bone in sham-operated rats were composed of fine particles. The trabecular bone area and trabecular thickness in OVX rats decreased by 50% from those in sham rats, these decreases were completely inhibited by administration of WSC at a concentration of 15 mg/kg/daily for 7 weeks. In this study, the mechanical strength in femur neck was significantly enhanced by the treatment of WSC for 7 weeks. In OVX rats, free T3 was normal in all cases, whereas free T4 was significantly increased. Although there was no difference between OVX and WSC-administered rats in T3 level, we have found significant difference between them in T4 level. These results strongly suggest that WSC is effective in preventing the development of bone loss induced by OVX in rats. |
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| Keywords: | Ovariectomy(OVX) High Molecular Weight Water-Soluble Chitosan(WSC) Serum Biochemistry Bone Histomorphometry |
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