天龙通心片对心肌缺血再灌注损伤及血液流变学的影响

目的:探索天龙通心片对大鼠心肌缺血再灌注损伤的保护作用,并观察其对大鼠血栓形成、血液黏度及家兔血小板聚集的影响。方法:取56只Wistar大鼠,除假手术组外,建立大鼠心肌缺血再灌注损伤模型,造模成功的模型分为模型组、合心爽组、复方丹参片组、天龙通心片4,2,1 g·kg~(-1)组,每组8只;硝基四氮唑蓝(N-BT)法观察受试药对心肌梗塞程度的改善作用;比色法检测受试药对血清超氧化物歧化酶(SOD),丙二醛(MDA)的影响。采用Chandler体外法检测正常组、天龙通心片4,2,1 g·kg~(-1)组、复方丹参片组、阿司匹林组大鼠体外血栓形成及血液黏度。Born氏比浊法观察正常组、天龙通心片2,1,0. 5 g·kg~(-1)组、复方丹参片组、阿司匹林组家兔血小板聚集变化水平。结果:与假手术组比较,模型组心肌梗死面积明显增大,血清SOD降低,MDA升高,均有显著性差异(P 0. 05);与模型组比较,天龙通心片各剂量组、合心爽组、复方丹参片组均能够减轻心肌梗塞程度,梗塞区占心室及心脏百分比降低,均有显著性差异(P 0. 05,P 0. 01);与正常组比较,天龙通心片4,2 g·kg~(-1)组、复方丹参片组、阿司匹林片组均能明显缩短血栓长度(P 0. 05,P 0. 01),明显减轻血栓湿重和干重(P 0. 05,P 0. 01);且明显降低切变率150,60,5 s~(-1)下的全血黏度(P 0. 05,P 0. 01);与正常组比较,天龙通心片2,1 g·kg~(-1)组、复方丹参片组、阿司匹林片组均能显著降低胶原,二磷酸腺苷(ADP),花生四烯酸(AA)诱导的家兔血小板聚集率(P 0. 05,P 0. 01)。结论:天龙通心片能保护大鼠心肌缺血再灌注损伤,并且具有抑制血小板聚集和血栓形成,降低血液黏度的作用。

Effect of Tianlong Tongxin Tablet on Myocardial Ischemia Reperfusion Injury and Hemorheology

Objective:To explore the protective effect of Tianlong Tongxin tablet on myocardial ischemia reperfusion injury in rats, and observe its effect on thrombosis, blood viscosity and platelet aggregation in rabbits. Method:Totally 56 Wistar rats were collected. Except for the sham operation group, all of the remaining rats were involved in the establishment of the rat myocardial ischemia reperfusion injury model. The successfully established model was divided model group, Hexinshuang group, compound Danshen tablet group and Tianlong Tongxin tablet groups (4, 2, 1 g·kg^-1). Nitrotetrazolium blue (N-BT) method was used to observe the alleviation of myocardial infarction. Colorimetry was used to detect the effect of the test drug on serum superoxide dismutase (SOD) and malondialdehyde (MDA). The Chandler in vitro method was used to detect thrombosis and blood viscosity in vitro of control group, Tianlong Tongxin tablets groups (4, 2, 1 g·kg^-1), compound Danshen tablets group and aspirin group. The Born turbidimetric method was used to observe the platelet aggregation levels of control group, Tianlong Tongxin tablets groups (2, 1, 0.5 g·kg^-1), compound Danshen tablets group and aspirin group. Result:Compared with the sham operation group, the myocardial infarction area, serum SOD and MDA in the model group were significantly increased (P<0.05). Compared with the model group, Tianlong Tongxin tablets groups, Hexinshuang group and compound Danshen tablets group could reduce the degree of myocardial infarction, and the percentage of infarction area in the ventricle and heart decreased significantly (P<0.05, P<0.01). Compared with the control group, Tianlong Tongxin tablets groups (4, 2 g·kg^-1), compound Danshen tablets group and Aspirin tablets group could significantly shorten the length of thrombosis (P<0.05, P<0.01), and significantly reduce the wet weight and dry weight of thrombosis (P<0.05, P<0.01). In addition, the whole blood viscosities under 150, 60 and 5 s^-1 shear rates were significantly reduced (P<0.05, P<0.01). Compared with the control group, the platelet aggregation rates induced by collagen, adenosine bisphosphate (ADP) and arachidonic acid (AA) in rabbits were significantly reduced in Tianlong Tongxin tablet group (2, 1 g·kg^-1), compound Danshen tablet group and Aspirin tablet group (P<0.05, P<0.01). Conclusion:Tianlong Tongxin tablet can protect rat myocardial ischemia reperfusion injury, inhibit platelet aggregation and thrombosis, and reduce blood viscosity.