PPARγ基因多态性与动脉粥样硬化性脑梗死的相关性研究

目的 探讨PPARγ基因多态性与动脉粥样硬化性脑梗死的关系.方法 本研究共纳入227例动脉粥样硬化性脑梗死患者和404例健康对照人群.以rs1875796为遗传标记,应用多聚酶链-限制性片段长度多态性(PCR-RFLP)技术检测PPARγ基因rs1875796的个体基因型.结果 女性动脉粥样硬化性脑梗死组rs1875796的C等位基因频率较对照组明显增高(χ~2=9.113,P=0.003,OR=2.211,95%CI 1.321～3.700),女性动脉粥样硬化性脑梗死rsl875796位点的CC+CT基因型频率较对照组明显增高(χ=8.032,P=0.005,OR=2.404,95%CI 1.310～4.411),经过多因素回归分析调整了传统危险因素的影响,两组间仍有显著性差异(P=0.006).而男性动脉粥样硬化性脑梗死组与对照组rs1875796的等位基因、基因型频率差异无显著意义.结论 PPARγ基因可能与女性动脉粥样硬化性脑梗死相关.

Abstract：

Objective To investigate the genetic association between the PPARγ gene and atherosclerotic cerebral infarction. Methods 227 patients with atherosclerotic cerebral infarction were recruited into this study, and 404 healthy people were as controls. SNP rs1875796,a C to T base change located in intron 4 of the gene,was used as a genetic marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs 1875796 ( Hha I site). Results The frequcncy of allele C was significantly higher in female patients than controls(χ~2 =9. 113,P =0. 003,OR =2.211,95% CI 1. 321～3.700). And the frequcncy of genotype CC + CT was also significantly higher in female patients than controls(χ~2 = 8.032,P = 0.005,OR =2.404, 95% CI 1.310～4.411). Multiple factor regression analysis showed that the differences was still significant after adjusting the traditional risk factors of atherosclerotic cerebral infarction. The frequency of allele C,and genotype CC + CT showed no significance between male patients and controls. Conclusions The present study suggests that the PPARγ gene is likely to contribute to the etiology of atherosclerotic cerebral infarction in female Chinese.

A population-based study of peroxisome proliferator-activated receptor-gamma gene for atherosclerotic cerebral infarction in a Chinese population

Objective To investigate the genetic association between the PPARγ gene and atherosclerotic cerebral infarction. Methods 227 patients with atherosclerotic cerebral infarction were recruited into this study, and 404 healthy people were as controls. SNP rs1875796,a C to T base change located in intron 4 of the gene,was used as a genetic marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs 1875796 ( Hha I site). Results The frequcncy of allele C was significantly higher in female patients than controls(χ~2 =9. 113,P =0. 003,OR =2.211,95% CI 1. 321～3.700). And the frequcncy of genotype CC + CT was also significantly higher in female patients than controls(χ~2 = 8.032,P = 0.005,OR =2.404, 95% CI 1.310～4.411). Multiple factor regression analysis showed that the differences was still significant after adjusting the traditional risk factors of atherosclerotic cerebral infarction. The frequency of allele C,and genotype CC + CT showed no significance between male patients and controls. Conclusions The present study suggests that the PPARγ gene is likely to contribute to the etiology of atherosclerotic cerebral infarction in female Chinese.