辛醇对红藻氨酸致痫大鼠的保护作用及其对海马组织连接蛋白43的影响

目的 研究辛醇对红藻氨酸(kainic acid,KA)致痫大鼠海马组织连接蛋白43(connexin43,CX43)表达的影响及其抗痫效应.方法 采用行为学评分评价KA致痫及辛醇腹腔注射后致痫大鼠的痫性发作程度和潜伏期,应用免疫组织化学方法检测海马CX43的表达.结果 KA组CX43的表达较正常对照组明显增高(P<0.01);辛醇组CX43的表达也较正常对照组明显增高(P<0.01),但较KA组明显下降.致痫后3h内辛醇组痫性发作评分较KA组明显下降(P<0.01),潜伏期明显延长(P<0.01).结论 反复痫性发作后CX43的表达增加.辛醇能够减少CX43表达,降低痫性发作的评分,延长痫性发作的潜伏期,具有较明显的抗癫痫效应.

Abstract：

Objective To study the effects of octanol on the expression of connexin 43 in the brain of epilepsy-rats induced by kainic acid and the anti-epileptic effect of octanol. Methods The epilepsy-rats induced by kainic acid and treated by octanol was assessed by behavior score. The expression of CX43 in the hippocampal tissue was measured by immuno-histochemistry. Results The expression of CX43 in the group of epilepsy-rats induced by kainic acid was higher than that in control group( P <0. 01) ;the expression of CX43 in the group treated by octanol was higher than that in control group( P <0. 01) ,but lower than the group of epilepsy-rats induced by kainic acid;the Patel score of the rats in the group treated by octanol was lower than the group only induced by kainic acid within 3 hours after the induction (P <0. 01) ,and the latency was longer (P <0.01). Conclusions After the epileptic seizure repeatedly, the expression of CX43 was upregulated. Octanol could reduce the expression of CX43, decrease the Patel score,and extend the latency of epileptic seizure. It has obviously anti-epileptic effect.

Protection of octanol on epilepsy-rats induced by kainic acid and the effects on the expression of connexin43 in hippocampal tissue

Objective To study the effects of octanol on the expression of connexin 43 in the brain of epilepsy-rats induced by kainic acid and the anti-epileptic effect of octanol. Methods The epilepsy-rats induced by kainic acid and treated by octanol was assessed by behavior score. The expression of CX43 in the hippocampal tissue was measured by immuno-histochemistry. Results The expression of CX43 in the group of epilepsy-rats induced by kainic acid was higher than that in control group( P <0. 01) ;the expression of CX43 in the group treated by octanol was higher than that in control group( P <0. 01) ,but lower than the group of epilepsy-rats induced by kainic acid;the Patel score of the rats in the group treated by octanol was lower than the group only induced by kainic acid within 3 hours after the induction (P <0. 01) ,and the latency was longer (P <0.01). Conclusions After the epileptic seizure repeatedly, the expression of CX43 was upregulated. Octanol could reduce the expression of CX43, decrease the Patel score,and extend the latency of epileptic seizure. It has obviously anti-epileptic effect.