| CSF-1对体外培养人牙囊细胞OPG表达的影响 |
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| 引用本文： | 孙海燕,林珠,金作林,张永宽. CSF-1对体外培养人牙囊细胞OPG表达的影响[J]. 实用口腔医学杂志, 2006, 22(2): 214-217 |
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| 作者姓名： | 孙海燕 林珠 金作林 张永宽 |
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| 作者单位： | 西安第四军医大学口腔医学院正畸科,710032 |
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| 摘 要： | 目的研究集落刺激因子(CSF-1)对体外培养的人牙囊细胞骨保护素(OPG)表达的影响。方法原代培养人牙囊细胞,传代至第3代,制备细胞爬片,进行OPG免疫组化染色;3~6代人牙囊细胞长至80%时,用胰酶消化,离心,重悬,制成单细胞悬液,接种到细胞培养皿,细胞长满至80%时,培养基中加入25ng/ml的CSF-1,孵育0.5、1、3、6h,分别收集上清液,ELISA法检测OPG蛋白分泌量的变化,提取总RNA进行RT-PCR,检测OPG mRNA的表达变化。结果正常人牙囊细胞OPG蛋白表达阳性;25ng/ml的CSF-1可降低人牙囊细胞OPG的表达,共同孵育1h,OPG表达降至最低(P<0.05)。结论牙囊细胞表达OPG,同时在牙齿萌出的特定时期,CSF-1通过下调人牙囊细胞OPG,减弱对破骨细胞形成的抑制作用,促进破骨细胞分化成熟,调节牙齿萌出。
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| 关 键 词： | 牙囊 集落刺激因子 骨保护素 牙齿萌出 |
| 文章编号： | 1001-3733(2006)-02-0214-04 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005-11-14 |
Regulation of osteoprotegerin expression by CSF-1 in human dental follicle cells |
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| Sun Haiyan, Lin Zhu, Jin Zuolin,et al.. Regulation of osteoprotegerin expression by CSF-1 in human dental follicle cells[J]. Journal of Practical Stomatology, 2006, 22(2): 214-217 |
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| Authors: | Sun Haiyan Lin Zhu Jin Zuolin et al. |
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| Affiliation: | Sun Haiyan, Lin Zhu, Jin Zuolin, et al. |
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| Abstract: | Objective:To study the effects of colony-stimulating factor-1 (CSF-1) on the expression of osteoprotegerin (OPG ) in human dental follicle cells.Methods:Human dental follicle cells culture was established and cell crawling slides were stained by OPG antibody. The third to sixth passage cells were incubated with 25 ng/ml CSF-1 for 0.5,1,3 and 6 h respectively,then the conditioned medium and cells were collected for ELISA and RT-PCR assay respectively.Results:OPG was expressed in the cytoplasm of human dental follicle cells.GSF-1 at 25 ng/ml and 1 h-exposure down-regulated OPG expression in the cells(P<0.05).Conclusion:OPG is located in human dental follicle cells,GSF-1 may down-regulate OPG expression in the cells. |
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| Keywords: | Dental follicle Colony-stimulating factor-1 Osteoprotegerin Tooth eruption |
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