腹部开放伤后海水浸泡致犬小肠缺血与缺血再灌注损伤的初步研究

目的 探讨腹部开放伤后海水浸泡致犬肠功能损伤的机制.方法 20只实验犬致伤后随机分为对照组(n=10)和海水浸泡组(n=10).对照组致伤后直接观察,海水浸泡组致伤后立即放人人工配制的海水中.于致伤前(0 h)及致伤后4、8、12、24 h测定实验犬的动脉血压和胃肠黏膜pH值.24 h后取小肠组织测定丙二醛(MDA)、一氧化氮/一氧化氮合酶(NO/NOS)、超氧化物歧化酶(SOD)含量.结果 海水浸泡组动脉血压明显下降,4 h后与对照组比较差异有统计学意义(P<0.05).海水浸泡组肠黏膜pH值在4 h后开始下降,与对照组比较差异有统计学意义(P<0.05);12 h升高,与对照组比较差异无统计学意义(P>0.05).海水浸泡组肠组织MDA、NO/NOS含量海水浸泡组与对照组比较均显著升高(P<0.05),SOD含量则明显降低(P<0.05).结论 肠道缺血和缺血再灌注损伤可能是海水浸泡导致实验犬肠损伤的原因之一.

Exploratory research on the effects of seawater immersion on the lesion of small intestine induced by ischemia-reperfusion in dogs following open chest injury

Objective To explore the mechanism of intestinal lesion induced by seawater immersion following open chest injury in dogs. Methods Twenty adult mongrel dogs were randomly divided into the control group (n = 10) and the seawater immersion group (n = 10). The control group was put under observation soon after abdominal injury. The seawater immersion group was immersed in manually prepared seawater immediately after abdominal injury. Arterial blood pressure and intestinal mucosa pH were monitored before injury (0 hour) and at hour4, 8, 12, and 24 after injury. Intestinal tissue samples were collected after 24 hours for the detection of MDA, NO/NOS and SOD. Results Arterial blood pressure for the animals of group B dropped significantly with statistical difference (P < 0.05), when it was compared with that of the control group. Intestinal mucosa pH of group B decreased at hour 4 with a marked difference, when it was compared with that of the control group (P < 0. 05). However, an increase was noted at hour 12 after immersion, with no significant difference, when a comparison was made between the 2 groups (P > 0. 05). Detection showed that levels of MDA, NO and NOS for the seawater immersion group increased obviously, when it was compared with that of the control (P < 0.05). Monitoring of intestinal SOD indicated that there was a clear reduction in the level of SOD for the seawater immersion group (P < 0. 05). Conclusions Trauma -induced ischemia and ischemia-reperfusion might be involved in lesion to the intestine of experimental dogs following seawater immersion.