苦参碱葡萄糖注射液合并丹参注射液对四氯化碳所致大鼠慢性肝损伤的保护作用

目的观察苦参碱葡萄糖注射液合并丹参注射液对慢性肝损伤的保护作用。方法将80只SD大鼠随机分为5组,即正常组、模型组、苦参碱葡萄糖注射液组、丹参注射液组和苦参碱葡萄糖注射液合并丹参注射液组各16只。正常组和模型组均给予0.9%氯化钠注射液20 mL/kg;苦参碱葡萄糖注射液组给予苦参碱葡萄糖注射液40 mL/kg;丹参注射液组给予丹参注射液1.6 mL/kg;苦参碱葡萄糖注射液合并丹参注射液组给予苦参碱葡萄糖注射液20 mL/kg＋丹参注射液0.8 mL/kg。应用四氯化碳（CCl4）攻击方法制备改进,大鼠皮下注射10%CCl4橄榄油溶液5 mL/kg,造成大鼠慢性肝损伤模型。观察造模大鼠存活率,进行病理组织学检查、血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、总胆红素（TBiL）和血清总蛋白（TP）、白蛋白（A）和白蛋白/球蛋白（A/G）的测定。结果苦参碱葡萄糖注射液组、丹参注射液组和苦参碱葡萄糖注射液合并丹参注射液组与模型组大鼠存活率比较差异均有统计学意义（P〈0.01）,高于模型组。正常组肝脏病理组织正常,仅2例肝细胞有轻度水肿或脂变;其余各组对CCl4所致慢性肝损伤大鼠肝脏病理组织学均有不同程度的影响。苦参碱葡萄糖注射液组、丹参注射液组和苦参碱葡萄糖注射液合并丹参注射液组治疗均能降低造模动物肝脏ALT、AST、ALP、TBiL水平,升高TP、A、A/G,其中以苦参碱葡萄糖注射液合并丹参注射液组效果明显。结论苦参碱葡萄糖注射液合并丹参注射液改善慢性肝损伤效果优于单独应用苦参碱葡萄糖注射液或丹参注射液。

Protective effect of matrine and glucose injection combined with Danshen injection against chronic hepatic damage induced by carbon tetrachloride in rats

Objective To observe the protective effect of matrine and glucose injection combined with Danshen injection on chronic hepatic damage.Methods 80 rats were randomly divided into five groups.They were control group（rats received saline）,model group（rats received 0.9% sodium chloride）,matrine and glucose injection group（rat received 40ml/kg matrine and glucose injection）,Danshen injection group（rat received 1.6 mL/kg Danshen injection）and matrine and glucose injection combined with Danshen injection group（rat received 20ml/kg matrine and glucose injection and 0.8ml/kg Danshen injection）.The model rats with chronic hepatic damage were induced by hypodermic injection of 10% carbon tetrachloride.Survival rate and histopathology were recorded.To observe the effect of matrine and glucose injection,danshen injection and matrine and glucose injection combined with danshen injection,ALT,AST,ALP,TBiL,TP,A and A/G were measured.Results Survival rate in matrine and glucose injection group,Danshen inection group and matrine and glucose injection combined with Danshen injection group were higher than that in model group（P〈0.01）.The levels of ALT,AST and TbiL in matrine and glucose injection group,Danshen injection group and matrine and glucose injection combined with Danshen injection group were higher than that in model group were decreased,and TP,A and A/G were increased in comparison with those in model group（P〈0.05）.The effect in matrine and glucose injection combined with Danshen injection group is the best in the three groups.Conclusion The effect of matrine and glucose injection combined with danshen injection on improving the chronic hepatic damage is better than a separate application of matrine and glucose injection or danshen injection.