| 应用多重PCR法对广东地区HBV进行基因型(A-F)分型 |
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| 引用本文： | 杨洁,戴琳,郭亚兵,杨守昌,王燕军,骆抗先. 应用多重PCR法对广东地区HBV进行基因型(A-F)分型[J]. 南方医科大学学报, 2002, 22(8): 707-709 |
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| 作者姓名： | 杨洁 戴琳 郭亚兵 杨守昌 王燕军 骆抗先 |
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| 作者单位： | 第一军医大学南方医院感染内科,广东,广州,510515;第一军医大学南方医院感染内科,广东,广州,510515;第一军医大学南方医院感染内科,广东,广州,510515;第一军医大学南方医院感染内科,广东,广州,510515;第一军医大学南方医院感染内科,广东,广州,510515;第一军医大学南方医院感染内科,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金(39800125),广东省自然科学基金(980223) |
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| 摘 要： | 目的建立乙型肝炎病毒(HBV)基因型(A-F)多重PCR分型方法,并利用该方法对广东地区HBV PCR阳性血清进行分型。方法将GenBank中114例HBV 全序列进行比较分析,找出每种基因型相对于其他五种基因型的独特序列,并根据这些独特序列设计出六对分别针对A-F基因型的特异引物。利用这六对引物建立HBV的多重PCR分型法。结果多重PCR与以前用PCR-限制温度长度多态型分析法的分型结果一致。对广州周边地区HBV携带者的初步分型结果显示,主要为B型和C型,分别占45.00%和38.75%,另外还有16.25%的D型。结论用多重PCR分型法准确易行,灵敏性高,便于推广应用。
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| 关 键 词： | 乙型肝炎病毒 基因型 多重聚合酶链反应 |
| 文章编号： | 1000-2588(2002)08-0707-03 |
| 修稿时间： | 2002-04-16 |
Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China |
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| YANG Jie,DAI Lin,GUO Ya-bing,YANG Shou-chang,WANG Yan-jun,LUO Kang-xian. Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China[J]. Journal of Southern Medical University, 2002, 22(8): 707-709 |
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| Authors: | YANG Jie DAI Lin GUO Ya-bing YANG Shou-chang WANG Yan-jun LUO Kang-xian |
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| Affiliation: | YANG Jie,DAI Lin,GUO Ya-bing,YANG Shou-chang,WANG Yan-jun,LUO Kang-xian Department of Infectious Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR. Method Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA. Result The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype. Conclusion This multiplex PCR method is simple, convenient and more differential. |
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| Keywords: | hepatitis B genotype multiplex polymerase chain reaction |
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