| 高原猪肝细胞生物反应器的生物学活性 |
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| 引用本文： | 张明森,李素芝,龙仁玲,杨光,江玉兰.高原猪肝细胞生物反应器的生物学活性[J].解放军预防医学杂志,2011,29(3):177-180. |
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| 作者姓名： | 张明森 李素芝 龙仁玲 杨光 江玉兰 |
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| 作者单位： | 西藏军区总医院高干病房,拉萨,850003 |
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| 基金项目： | 西藏自治区“十五”重点课题(No.2001-99) |
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| 摘 要： | 目的 了解在高原环境下,培养球形聚集体猪肝细胞中空纤维型生物反应器的生物学活性特征,为构建高原体外生物人工肝提供实验依据.方法 采用已适应本地高原环境的长白仔猪,并分离其肝细胞,经体外球形聚集体培养2 d后,移入中空纤维型生物反应器外腔中,内腔以培养液循环,继续培养10 d,观察培养细胞形态,检测外腔培养液中的总蛋白、...
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| 关 键 词： | 生物反应器 细胞培养 猪肝细胞 高原 生物人工肝 |
BIOLOGICAL ACTIVITY OF THE HOLLOW FIBER BIOREACTOR WITH CULTURED PORCINE HEPATOCYTE AT HIGH ALTITUDE |
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| ZHANG Ming-sen,LI Su-zhi,LONG Ren-ling,YANG Guang,JIANG Yu-lan.BIOLOGICAL ACTIVITY OF THE HOLLOW FIBER BIOREACTOR WITH CULTURED PORCINE HEPATOCYTE AT HIGH ALTITUDE[J].Journal of Preventive Medicine of Chinese People's Liberation Army,2011,29(3):177-180. |
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| Authors: | ZHANG Ming-sen LI Su-zhi LONG Ren-ling YANG Guang JIANG Yu-lan |
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| Institution: | ZHANG Ming-sen,LI Su-zhi,LONG Ren-ling,YANG Guang,JIANG Yu-lan(General Hospital of Tibet Military Command,Lasa 850003,China) |
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| Abstract: | Objective To understand the characteristic of biological activity of the hollow fiber bioreactor with cultured porcine hepatocytes at high altitude,so as to provide experimental basis for making up bioartificial liver at altitude.Methods Hepatocytes isolated from the porcine liver were cultured for 2 ds to form spheroid,then moved into the outside cavity of hollow fiber reactor and cultured for 10 ds.The shape of cultured cells was observed,and the total protein,albumin,urea and lactate dehydrogenase in cul... |
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| Keywords: | bioreactor cell culture porcine hepatocyte high altitude bioartificial liver |
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