ABC法检测慢性肝病患者骨髓细胞HBsAg、HBcAg和HBeAg的染色观察

用ABC法检测118例慢性肝病患者骨髓细胞HBsAg、HBcAg和HBeAg,阳性56例(47.5％)。其中单阳性31例(26.3％)、双阳性12例(10.2％)、3阳性13例(11.0％)。慢性乙型肝炎与肝硬变阳性率差异不显著,P>0.05,而肝癌与肝硬变、慢性乙型肝炎阳性率差异显著,P<0.05。结果表明骨髓细胞HBV感染与肝癌的发病率有密切关系。骨髓细胞分类计数,阳性骨髓细胞均在晚幼粒、杆状核及分叶核粒细胞系,其它骨髓细胞系均为阴性。

DETECTION OF HBsAg, HBcAg AND HBeAg IN MARROW CELLS OF THE PATIENTS WITH CHRONIC HEPATIC DISEASES

HBsAg, HBcAg and HBeAg in the marrow cells of 118 cases of patients with chronic hepatic diseases were determined with the ABC method. In 56 cases (47.5%) it was positive, and among them single, double and treble positives were 31 (26.3%), 12 (10.2%), 13 (11.0%), respectively. The difference of the positive rates between chronic hepatitis B and cirrhosis was not significant (P>0.05), while the differences between hepatocarcinoma and cirrhosis as well as hepatocarcinoma and hepatitis B were significant (P<0.05).The results indicate that the infection rate of the marrow cells by HBV is closely related to the incidence of hepatocarcinoma. The positive marrow cells consist mainly of the cell series of the metamyelocytes. band cells and polymorphonuelear granulocyte while the other marrow cells are not involved. This finding is significant in the prevention and treatment of chronic hepatic diseases.