癌源性免疫球蛋白G全分子相互作用蛋白质的质谱鉴定及其功能分析

目的鉴定与癌源性免疫球蛋白G（IgG）全分子相互作用的蛋白质，为研究其生物功能提供重要的线索。方法分别用兔
抗人IgG全分子抗体和兔IgG在宫颈癌细胞系HeLa中进行免疫共沉淀，将得到的免疫复合物经电泳分离后进行银染。经比较
分析后3条差异条带切下来做质谱鉴定，质谱数据经Swiss-Prot数据库分析。最后对所得的蛋白质进行筛选和功能注释分析。
结果最终得到6个可能与癌源性IgG全分子相互作用的蛋白质。结论通过免疫共沉淀结合质谱分析的方法最终得到了6个
可能与癌源性IgG全分子相互作用的蛋白质，为其功能研究提供了重要线索。

Functional analysis of cancer-derived immunoglobulin G whole molecule-interacting pro-teins identified by LC-MS/MS

Objective To identify cancer-derived immunoglobulin G (IgG) whole molecule-interacting proteins to provide
important clues for studying IgG biological functions. Methods HeLa cell lysate was immunoprecipitated with rabbit
antihuman IgG whole molecule antibody and normal rabbit IgG. The immunocomplex underwent sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE) and was detected with silver staining. Three prominently enhanced bands
were subjected to protein identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the MS data
were analyzed with Swiss-Prot database. Cancer-derived IgG whole molecule-interacting proteins were screened and
functionally annotated. Results and Conclusion We identified 6 potential cancer-derived IgG whole molecule-interacting
proteins with co-immunoprecipitation combined with LC-MS/MS, which provides valuable clues for studying the function of
cancer-derived IgG.