E-选择素在胚胎着床过程中小鼠子宫内膜的表达规律

目的：研究E-selectin在胚胎着床及早期分化中的作用。方法：用RT—PCR、Immunoblot、Western blot法检测小鼠胚胎着床前后蜕膜组织E-selectin的表达。结果：E-选择素在孕4天的小鼠子宫内膜中的表达出现升高，第5天达到峰值，随后妊娠第6、第7天E-selectin蛋白的表达则逐渐下降至正常水平。结论：提示E-选择素可能参与了胚胎着床过程。

Expression of E-selectin in the endometrium of mouse early pregnancy

Objective:To study the expression of E-selectin mRNA and protein in the endometrium of mouse early pregnancy.Methods: The protein of E-selectin was measured with Immunoblot and Western blot , mRNA with RT-PCR, agarose gel electrophoresis and followed by YDS image analysis.The PCR products was identified by restrict enzyme mapping. Results:The relative level of E-selectin protein in normal endometrium was 34.31 +6.68, dayl to day3 of early pregnancy were respectively 46.16 +2.37, 46.89 + 1.57 and 45.19 +7.55, compared with normal endometrium, had no significant differences ( P > 0.05). On the day4, day5, the expression of E-selectin protein were greatly higher than that of normal endometrium (57.52 + 7.03, 62.91 + 7.31; P < 0.05, P < 0.01), and then decreased to the normal level. The expression of E-selectin mRNA was the same with protein, but on dayl and day2 there were no mRNA expression , the surge was on day5, then decreased to normal level.Conclusion:The highest expression of E-selectin was on day4 and day5, coincided with the time embryo implantation. It is indicated that E-selectin may be have an effect on implantation.