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| 人CTLA-4Ig腺病毒载体的构建鉴定与表达 | |
| 引用本文： | 冯宁翰,张炜,吴军,眭元庚,钱立新,华立新,贺伟峰,宋宁宏,吴宏飞. 人CTLA-4Ig腺病毒载体的构建鉴定与表达[J]. 河北医学, 2005, 11(6): 481-483 |
| 作者姓名： | 冯宁翰 张炜 吴军 眭元庚 钱立新 华立新 贺伟峰 宋宁宏 吴宏飞 |
| 作者单位： | 1. 南京医科大学第一附属医院泌尿外科,江苏,南京,210029 2. 第三军医大学西南医院全军烧伤研究所,重庆,400038 |
| 摘 要： | 目的：运用PAdEasy系统构建了带荧光蛋白的人CTLA-4Ig腺病毒载体，为进一步研究CTLA-4Ig诱导免疫耐受的机制奠定了基础。方法：采用基因技术，将目的基因克隆至穿梭质粒pAdTrack-CMV中，利用PAdEasy系统进行重组，然后在293细胞中进行扩增。并进行病毒滴度测定。体外感染人肾小管上皮细胞。结果：酶切鉴定和PCR证明重组人CTLA-4Ig腺病毒载体构建正确。结论：应用PAdEasy细菌内重组技术成功构建了人CTLA-4Ig带荧光腺病毒载体。 |
| 关 键 词： | 腺病毒 CTLA-4Ig 同源重组 |
| 文章编号： | 1006-6233(2005)06-0481-03 |
The Express and Recombinant of Adenovirus of Human CTLA-4Ig Gene |
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| FENG Ning-han,ZHANG Wei,et al. The Express and Recombinant of Adenovirus of Human CTLA-4Ig Gene[J]. Hebei Medicine, 2005, 11(6): 481-483 | |
| Authors: | FENG Ning-han ZHANG Wei et al |
| Abstract: | Objective: To construct the recombinant adenovirus of human CTLA-4Ig gene .Method: The CTLA-4Ig gene was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination.Result: It is confirmed that the CTLA-4Ig gene were inserted successfully into the adenovirus by using restriction endonuclease and PCR analyses .Conclusion: The recombinant adenovirus of human CTLA-4Ig gene was successfully constructed. |
| Keywords: | Adenovirus CTLA-4 Homogenic reconstruction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |