| 高效毛细管电泳法测定白胡椒中胡椒碱的含量 |
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| 引用本文： | 李和光,周洪雷,蒋海强,杜昊忱,李飞. 高效毛细管电泳法测定白胡椒中胡椒碱的含量[J]. 辽宁中医杂志, 2010, 0(1): 134-135 |
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| 作者姓名： | 李和光 周洪雷 蒋海强 杜昊忱 李飞 |
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| 作者单位： | 山东中医药大学; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目(2007CB512601) |
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| 摘 要： | 目的:测定中药白胡椒中胡椒碱的含量。方法:采用高效毛细管电泳法,电泳条件为石英毛细管柱(50μm×90cm,实际长度81.5cm),运行缓冲液为0.02mol/LNa2HPO4:0.02mol/LNaH2PO4=95:5(16℃时pH=8.6),分离电压为28kV,压力进样30mbar×8s,温度为25℃,检测波长为343nm。结果:胡椒碱在0.05～0.30mg/mL范围内呈良好的线性关系(r=0.9995),平均回收率分别为99.82%,99.95%,100.20%;RSD分别为0.30%,0.69%,0.29%(n=3)。结论:高效毛细管电泳法可用于测定白胡椒中胡椒碱的含量。
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| 关 键 词： | 白胡椒 胡椒碱 高效毛细管电泳 |
Determination of Piperine in Fructus piperis by High Performance Capillary Electrophoresis |
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| LI He-guang,ZHOU Hong-lei,JIANG Hai-qiang,DU Hao-chen,LI Fei. Determination of Piperine in Fructus piperis by High Performance Capillary Electrophoresis[J]. Liaoning Journal of Traditional Chinese Medicine, 2010, 0(1): 134-135 |
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| Authors: | LI He-guang ZHOU Hong-lei JIANG Hai-qiang DU Hao-chen LI Fei |
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| Affiliation: | Shandong University of TCM/a>;Jinan 250355/a>;Shandong/a>;China |
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| Abstract: | Objective：To determine the content of Piperine in Fructus piperis.Methods：Apply the methods of HPCE,and the best condition was using the quartz capillary tube（50μm×90cm,practical length is 81.5cm）,0.02mol/L Na_2HPO_4：0.02mol/L NaH_2PO_4=95：5（pH=8.6 at 16℃）buffer,pressure injection 30mbar×8s,at a constant voltage of 28kV and temperature at 25℃.It was detected on 343nm.Results：Piperine had a good linearity within 0.05~0.30mg/mL（r=0.9995）,and the average recovery were 99.82%,99.95%,100.20% with RSD 0.30%,0.69%,0.29%（n=3）.Conclusion：This method is feasible for determination of Piperine in Fructus piperis. |
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| Keywords: | fructus piperis piperine HPCE |
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