2型糖尿病肾病患者血清同型半胱氨酸、叶酸和亚甲基四氢叶酸还原酶基因多态性的关系

目的:探究2型糖尿病肾病(DN)患者血清同型半胱氨酸(HCY)、叶酸(FOL)与亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C基因多态性的关系。方法:回顾性分析2017年1月至2018年12月于江苏盛泽医院确诊的2型糖尿病患者161例，其中DN患者81例[男41例，女40例，年龄(61.5±14.2)岁]、...

Relationship of serum homocysteine,folic acid and genetic polymorphism of methyltetrahydrofolate reductase in patients with type 2 diabetic nephropathy

Objective To investigate the relationship between serum homocysteine(HCY),folate(FOL)and methylenetetrahydrofolate reductase(MTHFR)C677T,A1298C gene polymorphism in patients with type 2 diabetic nephropathy(DN).Methods A total of 161 patients with type 2 diabetes diagnosed in Jiangsu Shengze Hospital between January 2017 and December 2018,including 81 DN(41 males,40 females;age:(61.5±14.2)years),80 diabetic mellitus without nephropathy(DM;42 males,38 females,age:(57.7±10.8)years),and 77 normal controls(NC;39 males,38 females,age:(58.2±16.3)years)were retrospectively analyzed.The serum levels of HCY and FOL were detected by enzyme circulation and electrochemiluminescence respectively.TaqMan genotyping technique was used to detect MTHFR 677T&A1298 gene polymorphism.The serum levels of HCY and FOL were compared with one-way analysis of variance(the least significant difference t test),and the distribution differences of MTHFR gene were analyzed byχ² test.Results The difference of HCY level among DN,DM and NC groups was significantly different((19.76±7.81),(15.62±5.01)and(8.09±3.74)μmol/L;F=81.738,P<0.001).The FOL level among the 3 groups was also significantly different((12.18±3.01),(13.50±2.71)and(15.43±2.95)μg/L;F=26.978,P<0.001).The frequencies of 677T allele(51.2%,83/162),677TT/1298AA genotype(25.9%,21/81)in DN group were significantly higher than those in DM(33.1%,53/160;11.2%,9/80)and NC(33.8%,52/154;10.4%,8/77)groups(χ² values:10.821,9.099,both P<0.05),but the 1298C allele frequency was not significantly different among the 3 groups(21.6%(35/162,DN)vs 16.9%(27/160,DM)vs 18.2%(28/154,NC);χ²=1.269,P>0.05).The levels of HCY and FOL in individuals with 677TT/1298AA genotype were significantly higher than those in individuals with other genotypes(F values:12.955,15.504,all P<0.05).Conclusion The abnormal metabolism of HCY and FOL caused by MTHFR C677T&A1298C gene polymorphism may be the genetic risk factor of DN.