健脾复方对人大肠癌移植瘤裸小鼠肿瘤细胞凋亡的影响

目的研究健脾复方对人大肠癌移植瘤裸小鼠肿瘤细胞凋亡的影响。方法将50只BALB/C裸小鼠建立人大肠癌原位移植瘤模型,并按1∶1∶1∶1∶1随机分入健脾复方组、塞来昔布组、氟尿嘧啶(5-Fu)组、模型对照组和空白对照组,分别治疗8周。采用免疫组化法检测凋亡相关的因子信号转导与转录激活因子-3(Stat-3)、B细胞淋巴瘤因子-2(Bcl-2)、存活素(Survivin)、半胱氨酸蛋白酶(Caspase)-3、Caspase-8和Caspase-9的表达。结果大肠癌肿瘤组织中Stat-3、Bcl-2、Caspase-9和Caspase-3的表达均存在异常。健脾复方能下调Stat-3、Bcl-2的表达,上调Caspase-9、Caspase-8、Caspase-3的表达,但对于Survivin的调节作用有待进一步确定。结论健脾复方能够调控大肠癌裸小鼠移植瘤的细胞凋亡,是其抑制大肠癌发生发展的可能机制。

Effect of Jianpi compound recipe on apoptosis in nude mice with human colorectal cancer grafts

Objective To investigate the effect of Jianpi compound recipe on apoptosis in nude mice with human colorectal cancer grafts.Methods Human colorectal cancer orthotopic transplanted tumor model was established in 50 BALB/C nude mice.The nude mice were randomized into Jianpi compound recipe group,celecoxib group,5-Fu group,model control group,and blank control group with a ratio of 1∶1∶1∶1∶1.All groups received respective treatments for 8 weeks.The expression of Stat-3,Bcl-2,Survivin,Caspase-9,Caspase-8,and Caspase-3 were assessed by immunohistochemistry method.Results The expressions of Stat-3,Bcl-2,Caspase 9,Caspase 8,and Caspase 3 were abnormal in colorectal cancer.Jianpi group showed down-regulation of Stat-3 and Bcl-2,up-regulation of Caspase-9,Caspase-8 and Caspase-3.The effect on Survivin needed to be further confirmed.Conclusion Jianpi compound recipe could regulate the apoptosis in nude mice with human colorectal cancer grafts,and this maybe the possible tumor-prevention mechanism of Jianpi compound recipe.