硫酸酯化茯苓多糖对裸鼠胃腺癌的抑制研究

目的探讨硫酸酯化茯苓多糖(S-PCS3-II)对裸鼠胃腺癌的抑制作用。方法无菌抽取人低分化胃腺癌细胞MKN-45,接种于裸鼠右侧腹股沟皮下,形成皮下移植瘤。将裸鼠随机分成5组:正常饮水饮食组、生理盐水(阴性对照)组、低剂量治疗组、高剂量治疗组、5-Fu(阳性对照)化疗组,从一般状况、大体病理、抑瘤率等方面观察硫酸酯化茯苓多糖对裸鼠胃腺癌的抑制作用。结果裸鼠胃腺癌模型制作成功率100%,硫酸酯化茯苓多糖(S-PCS3-II)能明显的抑制胃腺癌的生长,与阴性对照组比较,有显著性差异(P<0.05),高剂量组(250 mg/kg/d)的抑制率达到47.9%。结论硫酸酯茯苓多糖在裸鼠体内能有效地抑制胃腺癌细胞的增长,该多糖衍生物可以作为潜在的抗胃癌药物。

The inhibition effect study of sulfated derivatives polysaccharides from sclerotium of poria cocos for nude mice gastric adenocarcinoma

Objective To evaluate the inhibition effect of the sulfated derivatives polysaccharides from sclerotium of Poria cocos(S-PCS3-II) for nude mice gastric adenocarcinoma.Methods The MKN-45 cells of low differentiation gastric adenocarcinoma were extracted asepsistically and implanted subcutaneous layer of nude mice’s right lateral inguen in order to duplicated subcutaneous transplanted carcinoma,the nude mice animals were divided into five groups:regular group;saline water treatment group;low dose S-PCS3-II treatment group;high dose S-PCS3-II treatment group;5-Fu treatment group.The therapeutic effectiveness of the nude mice gastric tumor treated by S-PCS3-II were evaluated on general contitions,macropathology,tumor inhibition rate,et al.Results The rate was 100% of making the model of gastric tumor in nude mice.S-PCS3-II inhibited nude mice gastric tumor growth,with statistic significance was found between S-PCS3-II treatment groups and saline water treatment group(P<0.05),The inhibition rate was 47.9% for the high dose S-PCS3-II treatment group.Conclusion S-PCS3-II can inhibit the growth of gastric cancer cells in the nude mice.It might be a potential drug for anti-gastric cancer.