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Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance |
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| Authors: | Leung Hei Lehtinen Ulla Karjalainen Reijo Skinner Daniel Tooley Paul Leong Sally Ellingboe Albert |
| Affiliation: | (1) Department of Plant Pathology, University of Wisconsin, 53706 Madison, WI, USA;(2) USDA Foreign Disease-Weed Science Research Unit, 21701 Frederick, MD, USA;(3) USDA Plant Disease Resistance Research Unit, University of Wisconsin, 53706 Madison, WI, USA;(4) Present address: Department of Plant Pathology, Washington State University, 99164 Pullman, WA, USA;(5) Present address: Department of General Microbiology, University of Helsinki, Mannerheimintie 172, SF-00300 Helsinki, Finland |
| Abstract: | Summary Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences. |
| Keywords: | Transformation Hygromycin B resistance Rice blast fungus |
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