离体心脏灌流技术在抗心绞痛中药生物活性检测中的应用初探

目的:初步研究离体心脏灌流法在抗心绞痛中药生物活性测定中的应用。方法:用Langendorff法进行离体豚鼠心脏灌流,测定丹参注射液、冠心宁注射液、香丹注射液、注射用丹参、丹红注射液5个抗心绞痛中药注射剂在给药前后的心脏冠状动脉流量的变化。结果:以上5个注射剂在一定的浓度下均能扩张冠状动脉,增加冠脉流量,同一注射剂的不同批号在相同浓度下冠脉流量不一致。结论:Langendorff离体心脏灌流法很有可能成为检测抗心绞痛中药的生物活性的理想方法,值得进行深入研究。

Earliest Investigation on Application of Isolated Heart Perfusion Technique to Evaluate Bioactivity of Antianginal Chinese Medicine

Objective: To investigate the application of isolated heart perfusion technique to evaluate the bioactivity of antianginal Chinese medicine. Method: Isolated guinea pig heart perfusion was performed with Langendorff method to measure the change in perfusion volume of coronary pre and after administrating the 5 antianginal chinese medicines such as Danshen Injection, Guanxinnin Injection, Xiangdan Injection, Danhong Injection and Danshen for Injection. Result : All 5 injections above could dilate the coronary and increase the perfusion volume, but there was discrepancy in the increase of perfusion volume at the same dose of different lot number from same injection. Conclusion : It may be possible that Langendorff isolated heart perfusion technique would become a good method for evaluating the bioactivity of antianginal Chinese medicine and deserves further probe.